Effects of Cold Stress on the Functional Activity of Mouse Peritoneal Macrophages in Conditions of Opiate Receptor Blockade

We report here studies of the effects of 10- and 60-min cold stress on the formation of reactive oxygen species (ROS) and cytokine production by peritoneal macrophages and corticosterone levels in conditions of opiate receptor blockade. Exposure of mice to a temperature of –20°C for 10 min led to suppression of ROS production by macrophages, and this effect was prevented by administration of naloxone. In animals exposed to 60-min stress, conversely, naloxone-dependent stimulation of ROS production was seen. Neither variant of cold stress had any significant effect on interleukin-1β production. TNF-α production on the background of 60-min cooling was stimulated in a naloxone-dependent manner in both unstimulated and zymosan-stimulated cultures. Production of IL-10 by macrophages increased regardless of the duration of cold exposure, with and without stimulation, and stopped on the background of opiate receptor blockade. Thus, the direction of action of acute cold stress on macrophage function depended on the parameter measured and the duration of exposure; opiate receptor blockade significantly modified the immunoregulatory effects of cold.

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