Τρίτη, 4 Σεπτεμβρίου 2018

Identification and Characterization of Transcripts Regulated by Circadian Alternative Polyadenylation in Mouse Liver

Dynamic control of gene expression is a hallmark of the circadian system. In mouse liver, approximately 5-20% of RNAs are expressed rhythmically, and over 50% of mouse genes are rhythmically expressed in at least one tissue. Recent genome-wide analyses unveiled that, in addition to rhythmic transcription, various post-transcriptional mechanisms play crucial roles in driving rhythmic gene expression. Alternative polyadenylation (APA) is an emerging post-transcriptional mechanism that changes the 3'-ends of transcripts by alternating poly(A) site usage. APA can thus result in changes in RNA processing, such as mRNA localization, stability, translation efficiency, and sometimes even in the localization of the encoded protein. It remains unclear, however, if and how APA is regulated by the circadian clock. To address this, we used an in silico approach and demonstrated in mouse liver that 57.4 % of expressed genes undergo APA and each gene has 2.53 poly(A) sites on average. Among all expressed genes, 2.9 % of genes alternate their poly(A) site usage with a circadian (i.e., approximately 24 hours) period. APA transcripts use distal sites with canonical poly(A) signals (PASs) more frequently; however, circadian APA transcripts exhibit less distinct usage preference between proximal and distal sites and use proximal sites more frequently. Circadian APA transcripts also harbor longer 3'UTRs, making them more susceptible to post-transcriptional regulation. Overall, our study serves as a platform to ultimately understand the mechanisms of circadian APA regulation.



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Paramedic - Miller EMS

Miller EMS is filling PARAMEDIC positions at our Owasso, Oklahoma base. The Owasso base is a high volume transfer base handling not only routine transfers but, a high volume of transfers that challenge the paramedic's critical care skills on patients requiring care such as multiple IV infusions, ventilators, CPAP, BiPAP, chest tube maintenance, etc. In addition to this we are utilized as a 911 back-up ...

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Understanding value and how it relates to EMS costs

EMS should utilize a data-driven approach to value management that considers both quality and costs

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The role of the C‐terminus of SUR in the differential regulation of β‐cell and cardiac KATP channels by MgADP and metabolism

The Journal of Physiology, Volume 0, Issue ja, -Not available-.


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Understanding value and how it relates to EMS costs

EMS should utilize a data-driven approach to value management that considers both quality and costs

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Characterization of Highly Prevalent Plasmids Coharboring mcr-1, oqxAB, and blaCTX-M and Plasmids Harboring oqxAB and blaCTX-M in Escherichia coli Isolates from Food-Producing Animals in China

Microbial Drug Resistance, Ahead of Print.


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Plasma ferritin concentration is positively associated with in vivo fatty acid mobilization and insulin resistance in obese women

Experimental Physiology, Volume 0, Issue ja, -Not available-.


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Spotlight: Technimount System

Technimount System offers mounts for

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Critical Care Transport Paramedic – Vitalink - New Hanover Regional Medical Center

**Critical Care Transport Paramedic – Vitalink** Essential Responsibilities: · Provides high quality patient care and safe medical transport to sick and injured patients in all age groups · Uses an interdisciplinary model to work with the Airlink/Vitalink Staff Nurse and other appropriate medical personnel to establish and prioritize a plan of care consistent with approved treatment protocols, policies ...

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Advanced EMT - New Hanover Regional Medical Center

**Advanced EMT** The Advanced EMT provides **high quality patient care and safe medical transport to sick and injured patients in all age groups, primarily in a non-emergent setting**. · Patient care will be consistent with approved treatment protocols · Communicates and coordinates the patient's care in an interdisciplinary model

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Staff Nurse – Vitalink - New Hanover Regional Medical C

**Staff Nurse – Vitalink** The Staff RN **provides high quality patient care and safe medical transport to sick and injured patients in all age groups**. _Essential Responsibilities:_ · Collaborates with team and initiates care plan based on patient assessment and significant other input/data. · Continuously reviews and revises plan as needed per patient's condition. · Maintains thorough patient care ...

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Paramedic - EMS - New Hanover Regional Medical C

**Paramedic - EMS** Full-Time The Staff Paramedic provides **high quality patient care and safe medical transport to sick and injured patients in all age groups**. · Develops a plan of care for each patient that is consistent with approved treatment protocols, policies and procedures. · Responsible for the operation of emergency vehicles

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EMS From a Distance: Responding to Emergency

Emphasizing the old-world values of Squad 51 in today's EMS

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Multiplex PCR using YeaD and 16S rRNA gene to identify major pathogens in vibriosis of Litopenaeus vannamei

Abstract

The Vibrio species causing major diseases in Litopenaeus vannamei are Vibrio harveyi, Vibrio alginolyticus, and Vibrio parahaemolyticus. For multiplex PCR primers, YeaD was used to detect the three Vibrio species. Bioinformatic analysis such as MultiPLX and primer-BLAST was used to design stable and species-specific multiplex PCR primers. Multiplex PCR results showed clear band patterns with bands at 185 bp for V. alginolyticus, 396 bp for V. harveyi, 805 bp for V. arahaemolyticus, and 596 bp for common Vibrio species. The minimum concentration of DNA was measured by PCR; the value for V. alginolyticus was 0.1 ng, that of V. harveyi was 0.03 ng, and that of V. parahaemolyticus was 0.003 ng. Taken together, YeaD showed stability and specificity in identifying Vibrio species. Our multiplex PCR amplification method is an effective and inexpensive tool for identifying Vibrio species.



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A single-arm, phase II trial of neoadjuvant gemcitabine and S1 in patients with resectable and borderline resectable pancreatic adenocarcinoma: PREP-01 study

Abstract

Background

Neoadjuvant chemotherapy (NAC) represents a promising alternative to pancreatic ductal adenocarcinoma (PDAC) planned resection, but the survival impact remains undefined. To assess the feasibility and survival outcomes of NAC with gemcitabine and S1 (GS) for PDAC planned resection by prospective study.

Methods

Patients with resectable or borderline resectable PDAC received 2 cycles of NAC-GS and were offered curative resection followed by gemcitabine adjuvant. The primary endpoint was 2-year overall survival (OS). Adverse events during NAC, radiological and tumor marker responses, resection rate, and surgical safety were evaluated as secondary endpoints (UMIN000004148).

Results

We enrolled 104 patients between 2010 and 2012, with 101 patients treated using NAC-GS as the full analysis set (FAS). Of the 101 patients, 88% received the planned 2 cycles of NAC. Grade 3 neutropenia was common (35%). Radiological partial response and decreased carbohydrate antigen 19-9 concentration (> 50% decrease) were noted in 13% and 41%, respectively. R0/1 resections with M0 were performed in 65 patients without surgical mortality. Of the 65 patients, 44 received planned gemcitabine adjuvant for 6 months as the on-protocol cohort. The primary endpoint for the 2-year OS rate was 55.9% in the FAS (n = 101) and 74.6% in the on-protocol cohort (n = 44).

Conclusions

NAC-GS was feasible and actively prolonged survival following PDAC planned resection. Randomized control trials are needed to further clarify the survival benefit of NAC-GS in addition to surgery followed by adjuvant therapy.



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A phenomenological model of the time course of maximal voluntary isometric contraction force for optimization of complex loading schemes

Abstract

Purpose

The time course of maximal voluntary isometric contraction (MVIC) force is of particular interest whenever force capacities are a limiting factor, e.g., during heavy manual work or resistance training (RT) sessions. The objective of this work was to develop a mathematical model of this time course that is suitable for optimization of complex loading schemes.

Materials and methods

We compiled a literature overview of existing models and justified the need for a new model. We then constructed a phenomenological ordinary differential equation model to describe the time course of MVIC force during voluntary isometric contractions and at rest. We validated the model with a comprehensive set of published data from the elbow flexors. For this, we estimated parameters from a subset of the available data and used those estimates to predict the remaining data. Afterwards, we illustrated the benefits of our model using the calibrated model to (1) analyze fatigue and recovery patterns observed in the literature (2) compute a work–rest schedule that minimizes fatigue (3) determine an isometric RT session that maximizes training volume.

Results

We demonstrated that our model (1) is able to describe MVIC force under complex loading schemes (2) can be used to analyze fatigue and recovery patterns observed in the literature (3) can be used to optimize complex loading schemes.

Conclusions

We developed a mathematical model of the time course of MVIC force that can be efficiently employed to optimize complex loading schemes. This enables an optimal use of MVIC force capacities.



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The impact of dyspnea and threat of dyspnea on error processing

Psychophysiology, EarlyView.


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The association of garlic with Helicobacter pylori infection and gastric cancer risk: A systematic review and meta-analysis

Helicobacter

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HLA-DQA1/DRB1 polymorphism is associated with the development of hepatocellular carcinoma during entecavir treatment

Journal of Gastroenterology and Hepatology

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Cardiopulmonary fitness assessment on maximal and submaximal exercise testing in patients with Fabry disease

American Journal of Medical Genetics Part A, EarlyView.


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Ocular manifestations of Emanuel syndrome

American Journal of Medical Genetics Part A, EarlyView.


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Pain and sleep quality in children with non‐vascular Ehlers–Danlos syndromes

American Journal of Medical Genetics Part A, EarlyView.


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Mapping radiation distribution on ground based on the measurement using an unmanned aerial vehicle

Publication date: October 2018

Source: Journal of Environmental Radioactivity, Volumes 193–194

Author(s): Shuangyue Zhang, Ruirui Liu, Tianyu Zhao

Abstract

Mapping the radiation distribution on ground during a radiological emergency monitoring, or decontamination mission is an important task. Accurate knowledge of the radioactivity distribution can help radiation workers locate the contamination, which reduces unnecessary radiation exposure to personnel and perhaps to some extent also the exposure to the public. Recently, radiation monitoring systems based on unmanned aerial vehicles (UAV) have been widely studied and employed. However, development of algorithms for mapping the contamination from measured data obtained by the detection system mounted on an UAV is still lacking. In this work, we implemented an advanced statistical reconstruction algorithm for mapping spread and point radiation contamination. The algorithm significantly improves accuracy in the scope and location of radiation contamination.



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Altered Metabolic Profile of Triglyceride-Rich Lipoproteins in Gut-Lymph of Rodent Models of Sepsis and Gut Ischemia-Reperfusion Injury

Abstract

Background

Triglyceride-rich lipoproteins are important in dietary lipid absorption and subsequent energy distribution in the body. Their importance in the gut-lymph may have been overlooked in sepsis, the most common cause of critical illness, and in gut ischemia-reperfusion injury, a common feature of many critical illnesses.

Aims

We aimed to undertake an exploratory study of triglyceride-rich lipoprotein fractions in gut-lymph using untargeted metabolic profiling to identify altered metabolites in sepsis or gut ischemia-reperfusion.

Methods

The gut-lymph was collected from rodent sham, sepsis, and gut ischemia-reperfusion models. The triglyceride-rich lipoprotein-enriched fractions isolated from the gut-lymph were subjected to a dual metabolomics analysis approach: non-polar metabolite analysis by ultra-high performance liquid chromatography–mass spectrometry and polar metabolite analysis by gas chromatography–mass spectrometry.

Results

The metabolite analysis of gut-lymph triglyceride-rich lipoprotein fractions revealed a significant increase (FDR-adjusted P value < 0.05) in myo-inositol in the sepsis group and monoacylglycerols [(18:1) and (18:2)] in gut ischemia-reperfusion. There were no significantly increased specific metabolites in the lipoprotein-enriched fractions of both sepsis and gut ischemia-reperfusion. In contrast, there was a widespread decrease in multiple lipid species in sepsis (35 out of 190; adjusted P < 0.05), but not in the gut ischemia-reperfusion.

Conclusions

Increased levels of myo-inositol and monoacylglycerols, and decreased multiple lipid species in the gut-lymph triglyceride-rich lipoprotein fraction could be candidates for new biomarkers and/or involved in the progression of sepsis and gut ischemia-reperfusion pathobiology.



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Concise Commentary: Quercetin Flavonoid of the Month or IBD Therapy?



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Intracellular Ca 2+ mobilization pathway via bradykinin B 1 receptor activation in rat trigeminal ganglion neurons

Abstract

Bradykinin (BK) and its receptors, B1 and B2, in trigeminal ganglion (TG) neurons are involved in the regulation of pain. Recent studies have revealed that B1 receptors are expressed in neonatal rat TG neurons; however, the intracellular signaling pathway following B1 receptor activation remains to be elucidated. To investigate the mechanism by which B1 receptor activation leads to intracellular Ca2+ mobilization, we measured the intracellular free Ca2+ concentration ([Ca2+]i) in primary-cultured TG neurons. The application of Lys-[Des-Arg9]BK (B1 receptor agonist) increased the [Ca2+]i in these TG neurons even in the absence of extracellular Ca2+. Pretreatment with inhibitors of ryanodine receptors or sarco/endoplasmic reticulum Ca2+-ATPase suppressed the increase in Lys-[Des-Arg9]BK-induced [Ca2+]i. The Lys-[Des-Arg9]BK-induced [Ca2+]i increase was unaffected by phospholipase-C inhibitor. B1 receptor activation-induced [Ca2+]i increase was suppressed by phosphodiesterase inhibitor and enhanced by adenylyl cyclase inhibitor. These results suggest that B1 receptor activation suppresses intracellular cAMP production via adenylyl cyclase inhibition and mobilizes intracellular Ca2+ via ryanodine receptors that access intracellular Ca2+ stores.



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A case of chronic pancreatitis exacerbation associated with pancreatic arteriovenous malformation: a case report and literature review

Abstract

A 60-year-old man with an unruptured cerebral aneurysm and family history of moyamoya disease was admitted to our hospital with epigastric pain since the previous day. Serum levels of pancreatic enzyme were elevated and abdominal contrast-enhanced computed tomography showed localized enlargement of the pancreatic tail in the arterial phase and revealed numerous areas of fine mesh-like vascular hyperplasia consistent with an enlarged pancreatic tail. We diagnosed pancreatic arteriovenous malformation (P-AVM) with acute pancreatitis. Furthermore, in the pancreatic body, endoscopic ultrasonography showed lobularity (honeycombing type) and hyperechoic foci (non-shadowing), which suggests chronic pancreatitis. Acute management was performed with conservative treatment including administration of replacement fluids and proteolytic enzyme inhibitor. Distal pancreatectomy for P-AVM was performed because P-AVM is associated with acute pancreatitis recurrence, development of portal hypertension, progression of chronic pancreatitis, and refractory duodenal bleeding. Histological findings on the resected specimens revealed the anastomosis of abnormal arteries and veins, which suggested P-AVM. In addition, inflammation accompanied by fat necrosis due to ischemic infarction in the pancreatic tail, which suggested acute pancreatitis, and mild fibrosis in the pancreatic body, which suggested chronic pancreatitis, were shown. Although P-AVM is associated with various complications, symptomatic P-AVM should be considered a chronic and progressive disease.



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Age-related reversal of spinal excitability during anticipatory postural control

Abstract

Introduction

An internal perturbation of standing balance activates muscles critical for maintaining balance and is preceded by anticipatory postural adjustments (APAs). In healthy younger adults, a measure of spinal excitability in the form of the Hoffmann (H) reflex becomes depressed during APAs but how aging affects the reflex control of APAs is unknown.

Methods

We compared H reflex excitability profiles in the right soleus muscle, indirectly indicating APA, between younger (n = 11, age 19–24 years), middle-aged (n = 10, age 37–56 years), and older healthy adults (n = 11, age 63–78 years). Subjects rapidly raised the right-dominant arm in response to an auditory cue. The H reflex was evoked 120 ms, 100 ms, 80 ms, 60 ms, 40 ms, 20 ms, and 0 ms before as well as 20 ms after the onset of the right anterior deltoid muscle activation. For data processing, each trial was controlled for the corresponding background EMG activity before normalizing the standing data to the data in sitting in the 8 time bins.

Results

All subjects showed a silent period in the soleus background electromyographic activity, suggesting the presence of APA. We found that the stereotypical H reflex depression associated with APAs in younger adults was reduced in middle-aged adults and reversed to facilitation in older adults. The depression occurred in 10 out of 11 younger adults, whereas all 11 older adults exhibited facilitation.

Conclusion

Because APAs are organized at the supraspinal level, we speculate a supraspinal origin of the age-related reflex facilitation during APAs.



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Toward standardized patient positioning to avoid peripheral nerve injury during laparoscopic colorectal surgery: Application of a protocol and study of the resulting relationship between total rotation time and contact pressure at the shoulder

Asian Journal of Endoscopic Surgery, EarlyView.


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Exploring Pain Management Among Asian Immigrants with Chronic Pain: Self-Management and Resilience

Abstract

Asians immigrants (AIs) are one of the fastest growing racial groups in many countries globally. Despite pain prevalence, studies on chronic pain management among AIs is limited in the literature. An integrative review was conducted exploring the current state of science on chronic pain management among AIs. Several databases were used to identify related articles and 15 studies met the inclusion criteria. Two major themes emerged: (a) self-management, pertaining to how AIs take responsibility for their pain, and (b) resilience, their adaptive behaviors indicating low levels of pain-related dysfunction and burden despite chronic pain severity. Resilience plays a significant role in the mechanism by which self-management works in pain among AIs. Chronic pain management is a complex process where challenges to effective treatments exist. Findings have significant implications to healthcare providers and the general pain population. Future research directions include the necessity for increased participation of AIs in studies.



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