Accuracy and Reliability of Assessing Lateral Compartmental Leg Composition Using DXA.

Purpose: Investigate the accuracy and reliability of a novel dual X-ray absorptiometry (DXA) scanning method in the frontal plane for total, fat, and lean mass quantification of the anterior and posterior upper leg compartments. Methods: Twenty-one (11 female; X[Combining Overline]age=20.3+/-1.3 yrs) college athletes were assessed for total and regional body composition using DXA. Segmentation of anterior/posterior thigh compartments was measured with participants lying on their right and left sides and the scanned leg elevated with two foam pads. Custom regions of interest (ROIs) were created manually with enCoreTM software for each scan using bony landmarks to quantify lean, fat, and total masses. Paired t-tests assessed this novel positioning method's accuracy against standard positioning. Intra-class correlations (ICCs) and coefficients of variation (CV) examined inter- and intra-rater reliability for lateral scan measures of total, fat, and lean masses from manually created ROIs. Results: All mean differences (+/-SD) between frontal and lateral DXA scans of right (R) and left (L) leg total mass (R: 8.42+/-195.57 g; L: 19.47+/-131.80 g), fat mass (R: 61.26+/-215.66 g; L: -5.89+/-239.97 g), and lean mass (R: -103.00+/-302.54 g; L: -27.58+/-288.14 g) were non-significant (p-value range: 0.15 to 0.91). ICCs were high for all composition measures between- and within-raters, ranging from 0.983 to 0.999 and 0.954 to 0.999, respectively, with low variation across measures (all CVs:

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