RESISTANCE EXERCISE SELECTIVELY MOBILIZES MONOCYTE SUBSETS: ROLE OF POLYPHENOLS

Purpose To examine the impact of polyphenol supplementation on the recruitment, mobilization and activation of monocyte subsets following resistance exercise. Methods Thirty-eight recreationally active males (22.1 ± 3.1 yrs; 173.9 ± 7.9 cm; 77.8 ± 14.5 kg) were assigned to 28-days of polyphenol supplementation (PPB), placebo (PL) or control (CON). Blood samples were obtained before (PRE), immediately-(IP), one-(1H), five-(5H), 24-(24H) and 48-(48H) hours post-resistance exercise (PPB/PL) or rest (CON). Fine-needle biopsies were obtained from the vastus lateralis at PRE, 1H, 5H and 48H. Circulating concentrations of macrophage chemoattractant protein-1 (MCP-1) and fractalkine, as well as intramuscular MCP-1 were analyzed via multiplex assay. Changes in the proportions and expression of CD11b on monocyte subsets were assessed via flow cytometry. Results Circulating MCP-1 increased in PPB and PL at IP with further increases at 5H. Intramuscular MCP-1 was increased at 1H, 5H and 48H in all groups. Classical monocyte proportions were reduced in PPB and PL at IP, and increased at 1H. Nonclassical monocytes were increased in PPB and PL at IP, while intermediate monocytes were increased at IP, and reduced at 1H. Intermediate monocytes were increased in PPB at 24H and 48H. CD11b expression was reduced on PPB compared to PL and CON at PRE on intermediate and nonclassical monocytes. Conclusions Resistance exercise may elicit selective mobilization of intermediate monocytes at 24H and 48H, which may be mediated by tissue damage. Additionally, polyphenol supplementation may suppress CD11b expression on monocyte subsets at rest. Author Contributions:Conception and design of research: ARJ, JRH, JRT, KSB, DDC, KAH, JRS; Acquisition of data: ARJ, JRT, KSB, ANV, DDC, LPO; Data analysis and interpretation: ARJ, JRH, DDC, DHF; Manuscript draft and revision: ARJ, JRH, KAH SRA, DHF, JRS; Approval of final version: ARJ, JRH, JRT, KSB, ANV, DDC, LPO, KAH, SRA, DHF, JRS. Clinical Trial Registration: NCT02442245 Corresponding Author: Adam R. Jajtner, PhD. Department of Exercise Physiology; Kent State University, 350 Midway Dr.; Kent, OH 44242, Phone: 330-672-0212; Fax: 330-672-2250. ajajtner@kent.edu Funding: Kemin Foods, L.C. Conflict of Interest: KAH is employed by Kemin Foods, LC. All other authors report no actual or potential conflicts of interest. Accepted for Publication: 18 June 2018 © 2018 American College of Sports Medicine

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