BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF LOSARTAN IN RABBIT PLASMA BY RP-HPLC

2016-11-08T03-09-50Z
Source: Indo American Journal of Pharmaceutical Research
Sandhya Pamu*, , Subrahmanyam C V S , Rao Patnaik K S K .
A simple and selective RP-HPLC method was developed for the determination of Losartan potassium in rabbit plasma. Chromatographic separation of Losartan was achieved on a Thermosil ODS C-18 column (150×4.6mm) 5μ particle size, using mobile phase consisting of a mixture of Phosphate buffer and Acetonitrile (60/40 v/v, pH 4) at 245nm. Linearity was observed in the range of 20-150 ng/mL for Losartan potassium with a mean correlation coefficient of 0.999. The accuracy of the method was assessed by recovery studies at three different levels. Recovery studies indicated that the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing % RSD less than 2. All statistical data proved validity of the method and can be used for pharmacokinetic analysis of Losartan in rabbit plasma.


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