Evaluation of Helicobacter pylori infection and clarithromycin resistance in strains from symptomatic Colombian children

Objectives: To determine the current prevalence of Helicobacter pylori in symptomatic Colombian children and evaluate the presence of mutations associated with clarithromycin resistance. Patients and Methods: Biopsies from 133 children were analyzed. The gastric fragment was used for urease test and reused for PCR-sequencing of the 23SrDNA gene. Mutations were detected by bioinformatic analysis. Results: PCR-sequencing established that H. pylori infection was present in 47% of patients. Bioinformatics analysis of the 62 positive sequences for 23SrDNA revealed that 92% exhibited a genotype susceptible to clarithromycin, whereas remain strains (8%) showed mutations associated with clarithromycin resistance. Conclusions: The low rate of resistance to clarithromycin (8%) suggests that conventional treatment methods are an appropriate choice for children. Recycling a biopsy that is normally discarded reduces the risks associated with the procedure. The 23SrDNA gene amplification could be used for a dual purpose: detection of H. pylori and determination of susceptibility to clarithromycin. Address correspondence and reprint requests to María de Pilar Delgado, Department of Biological Sciences, Universidad de los Andes, Bogotá, Colombia, Cra 1 N(18A- 10 Office J211, Zip Code 111711 (e-mail: mdelgado@uniandes.edu.co). Received 28 July, 2017 Accepted 12 April, 2018 Conflicts of Interest and Source of Funding. This work was supported by Universidad de Los Andes and Fundación Santa Fe de Bogotá University Hospital, Center for Studies and Health Research (CEIS), Bogotá (5th Joint Research Call, 2011). Code:CU1010100. No conflicts of interest to declare. Author roles Yuliet Liliana Rosero Lasso. Contributed to the study design, acquisition and data analysis, writing, revision and final approval of the manuscript. Additionally, she has agreed to be accountable for all aspects of the work and ensures the integrity of the study. Betsy Verónica Arévalo-Jaimes. Contributed in data analysis, writing, revision and final approval of the manuscript. Additionally, she has agreed to be accountable for all aspects of the work and ensures the integrity of the study. María de Pilar Delgado Contributed to the study design, data analysis, revision and final approval of the manuscript. Additionally, she has agreed to be accountable for all aspects of the work and ensures the integrity of the study. José Fernando Vera-Chamorro. Contributed to the study design and data acquisition, revision and final approval of the manuscript. Additionally, he has agreed to be accountable for all aspects of the work and ensures the integrity of the study. Daniella García. Contributed to the study design, revision and final approval of the manuscript. Additionally, she has agreed to be accountable for all aspects of the work and ensures the integrity of the study. Andrea Ramírez. Contributed to the study design, collection and data analysis, revision and final approval of the manuscript. Additionally, she has agreed to be accountable for all aspects of the work and ensures the integrity of the study. Paula A Rodríguez-Urrego. Contributed in the analysis of samples, revision and final approval of the manuscript. Additionally, she has agreed to be accountable for all aspects of the work and ensures the integrity of the study. Johanna Álvarez. Contributed in the analysis of samples, revision and final approval of the manuscript. Additionally, she has agreed to be accountable for all aspects of the work and ensures the integrity of the study. Carlos Alberto Jaramillo. He participated in the study design, revision and final approval of the manuscript. Additionally, he has agreed to be accountable for all aspects of the work and ensures the integrity of the study. © 2018 by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology,

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