Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, Aedes aegypti [Genetics]

The development of CRISPR/Cas9 technologies has dramatically increased the accessibility and efficiency of genome editing in many organisms. In general, in vivo germline expression of Cas9 results in substantially higher activity than embryonic injection. However, no transgenic lines expressing Cas9 have been developed for the major mosquito disease vector Aedes...

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