Exercise serum increases glut4 in human adipocytes

NEW FINDINGS

What is the central question of this study?

Do circulating factors mediate exercise‐induced effects on adipose tissue GLUT4 expression?

What is the main finding and its importance?

Serum (10%) obtained from human volunteers immediately after a single exercise bout increased GLUT4 protein levels in human adipocytes in culture. This result suggests that circulating factors may mediate exercise effects on adipose tissue GLUT4 and prompt further effort to identify the specific factor(s) and tissue(s) of origin.

Abstract

In this study we tested the hypothesis that circulating factors generated during exercise increase adipose tissue GLUT4 expression. Serum was obtained from eight healthy subjects before and after 60 min of cycling exercise and primary adipocytes were cultured from stromal vascular fractions that were isolated from subcutaneous abdominal adipose tissue samples from two healthy, male volunteers. Exposure of human primary adipocytes to 10% serum obtained after exercise for 48 hr increased GLUT4 protein expression, on average, by 12% compared with exposure to 10% serum obtained at rest before exercise. GLUT4 mRNA levels were increased after 12 hr of exposure to exercise serum but were unchanged after 6 and 24 hr of exposure. Our results suggest that circulating factors may mediate exercise effects on adipose tissue GLUT4 expression and encourage further efforts to identify the potential factor(s), tissue(s) of origin and physiological relevance.

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