ATP Production of Muscle Mitochondria after Acute Exercise in Lean and Obese Humans

Introduction Current evidence indicates mitochondrial dysfunction in humans with obesity. Acute exercise appears to enhance mitochondrial function in muscle of non-obese humans, but its effects on mitochondrial function in muscle of humans with obesity are not known. We sought to determine whether acute aerobic exercise stimulates mitochondrial function in subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria in humans with obesity. Methods We assessed maximal ATP production rate (MAPR) and citrate synthase (CS) activity in isolated SS and IMF mitochondria from subjects with BMI 32 kg/m2 (median age 29 years, IQR 20-39 years) before and 3 hours after a 45-min cycling exercise at an intensity corresponding to 65% heart rate reserve. SS and IMF mitochondria were isolated from muscle biopsies using differential centrifugation. MAPR and CS activity were determined using luciferase- and spectrophotometric enzyme-based assays, respectively. Results Exercise increased MAPR in IMF mitochondria in both non-obese subjects and subjects with obesity (P 0.05). Exercise increased MAPR supported by complex II in SS mitochondria, in both groups (P 0.05). CS specific activity increased in SS mitochondria in response to exercise only in non-obese subjects (P

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