Purpose This investigation evaluated myocellular responses to an integrated resistance and aerobic training program (SPRINT) during 70 days of bed rest. Methods Training was six days per week on a small-footprint gravity-independent flywheel resistance and aerobic device (FLY); three days of maximal flywheel supine quadriceps and calf exercises with continuous rowing separated by 4-6 hours, and three days of interval rowing. Vastus lateralis (VL) and soleus (SOL) muscle biopsies were obtained from eight healthy males (age=28±4 y, BMI=25±3 kg•m−2, VO2max=42±6 ml•kg−1•min−1) before and after 6° head-intrdown tilt bed rest. VL and SOL myosin heavy chain (MHC) I and IIa single muscle fiber size and functional characteristics, as well as overall fiber type distribution, capillarization, and metabolic enzyme activities were evaluated. Results In the VL, MHC I size and power (absolute and normalized) were preserved. MHC IIa fibers hypertrophied (+6%, P
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