Utility and reliability of non-invasive muscle function tests in high-fat fed mice

Abstract

Non-invasive tests are commonly used for assessing muscle function in animal models. The value of these tests in obesity, a condition where muscle strength is reduced, is unclear. We investigated the utility of three non-invasive muscle function tests, namely grip strength (GS), hang wire (HW), and four-limb hanging (FLH) in C57BL/6 mice fed chow (n = 48) or high-fat diet (HFD, n = 48) for 20 weeks. Muscle function tests were performed at 5, 10, and 20 weeks. After 10 and 20 weeks, HFD mice had significantly reduced GS (10 weeks chow:1.89 ± 0.1, HFD:1.79 ± 0.1; 20 weeks chow:1.99 ± 0.1, HFD:1.75 ± 0.1N), FLH (10 weeks chow:2552 [1337–4964], HFD:1230 [749–1994]; 20 weeks chow:2048 [765–3864], HFD:1036 [717–1855] seconds*gram body weight) and HW reaches (10 weeks chow:4 [2–5], HFD:2 [1–3]; 20 weeks chow:3 [1–5], HFD:1 [0–2] (n)) and higher falls (10 weeks: chow:0 [0–2], HFD:3 [1–7]; 20 weeks chow:1 [0–4], HFD:8 [5–10] (n)). GS was reliable in both dietary groups (Intraclass Correlation Coefficient (ICC) = 0.5–0.8; P < 0.05) whereas FLH showed good reliability in chow (ICC = 0.7; P < 0.05) but not in HFD mice after 10 weeks (ICC < 0.5). Our data demonstrate that non-invasive muscle function tests are valuable and reliable tools for assessment of muscle strength and function in high-fat fed mice.

This article is protected by copyright. All rights reserved

from Physiology via xlomafota13 on Inoreader http://ift.tt/2prR8sy
via IFTTT