Temporal and spatial expression pattern of Nnat during mouse eye development

Publication date: Available online 27 December 2016
Source:Gene Expression Patterns
Author(s): Saadettin Sel, Eva Patzel, Lucia Poggi, Delia Kaiser, Thomas Kalinski, Martin Schicht, Friedrich Paulsen, Norbert Nass
BackgroundNeuronatin (Nnat) was initially identified as a highly expressed gene in neonatal mammalian brain. In this study, we analyze the spatial and temporal expression pattern of Nnat during mouse eye development as well as in the adult.MethodsThe expression of Nnat was analyzed on mRNA as well as protein level. The presence of Nnat transcripts in the adult retina was examined using reverse transcription-polymerase chain reaction (RT-PCR). Nnat protein expression was evaluated by Western blot and immunohistochemistry during eye development at embryonic day (E) 12, 15, 16 and postnatal day (P) 7, 14, 30 and 175 (adult).ResultsImmunohistochemical studies of the developing mouse eye revealed Nnat expression in embryonic and adult neuroretina as well as in corneal epithelial, stromal, endothelial cells and in lens epithelium. Expression of Nnat was detected from E12 onwards and was also present in adult eyes.ConclusionsThe expression pattern suggests that Nnat may play an important role during eye development and in the maintenance of mature eye.



from Genetics via xlomafota13 on Inoreader http://ift.tt/2ieaydL
via IFTTT