Influence of training intensity on adaptations in acid/base transport proteins, muscle buffer capacity, and repeated-sprint ability in active men

McGinley C, Bishop DJ. Influence of training intensity on adaptations in acid/base transport proteins, muscle buffer capacity, and repeated-sprint ability in active men. J Appl Physiol 121: 1290–1305, 2016. First published October 14, 2016; doi:10.1152/japplphysiol.00630.2016.—This study measured the adaptive response to exercise training for each of the acid-base transport protein families, including providing isoform-specific evidence for the monocarboxylate transporter (MCT)1/4 chaperone protein basigin and for the electrogenic sodium-bicarbonate cotransporter (NBCe)1. We investigated whether 4 wk of work-matched, high-intensity interval training (HIIT), performed either just above the lactate threshold (HIIT20; n = 8), or close to peak aerobic power (HIIT90; n = 8), influenced adaptations in acid-base transport protein abundance, nonbicarbonate muscle buffer capacity (βm_{in vitro}), and exercise capacity in active men. Training intensity did not discriminate between adaptations for most proteins measured, with abundance of MCT1, sodium/hydrogen exchanger (NHE) 1, NBCe1, carbonic anhydrase (CA) II, and CAXIV increasing after 4 wk, whereas there was little change in CAIII and CAIV abundance. βm_{in vitro} also did not change. However, MCT4 protein content only increased for HIIT20 [effect size (ES): 1.06, 90% confidence limits x / ÷ 0.77], whereas basigin protein content only increased for HIIT90 (ES: 1.49, x / ÷ 1.42). Repeated-sprint ability (5 x 6-s sprints; 24 s passive rest) improved similarly for both groups. Power at the lactate threshold only improved for HIIT20 (ES: 0.49; 90% confidence limits ± 0.38), whereas peak O₂ uptake did not change for either group. Detraining was characterized by the loss of adaptations for all of the proteins measured and for repeated-sprint ability 6 wk after removing the stimulus of HIIT. In conclusion, 4 wk of HIIT induced improvements in each of the acid-base transport protein families, but, remarkably, a 40% difference in training intensity did not discriminate between most adaptations.

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