Effects of a Supernatant of Fetal Neurogenic Cells on Proliferative Activity in Glioma C6 Cell Culture

We estimated the effects of a rat fetal neurogenic cell supernatant (RFNCS) on proliferative activity of cultured cells of rat brain glioma (C6 cell line). The RFNCS was obtained from a cell suspension of the brains from rat fetuses (gestation day 14, E14). We added 0.10 mg/ml of RFNCS to the culture medium of experimental cultures and incubated the latter for 48 h. Immunocytochemical staining with respect to the Ki-67 proliferation marker was performed using rabbit monoclonal antibodies against this protein. In glioma C6 cell cultures, we observed degenerating and necrobiotically modified tumor cells with rounded cell bodies and reduction of the processes. Under the action of RFNCS, the mean density of the cells in 10 test fields of vision in experimental cultures (0.04 mm²) became significantly smaller than that in the control (332.0 ± 36.0 vs. 569.5 ± 70.5; P = 0.00026); the mean value of the nuclear/cytoplasmic ratio in malignant cells mildly (insignificantly) decreased (0.28 ± 0.01 vs. 0.32 ± 0.02; P = 0.64) and the share of cells with multiple nucleoli was more than two times smaller (3.53 ± 0.33% and 7.97 ± 0.25%, respectively; P = 0.053). The mitotic index of cultured malignant cells subjected to the action of RFNCS became four times lower (1.10 ± 0.04% vs. 4.90 ± 0.09%; P = 0.009), while the share of malignant cells immunopositive with respect to Ki-67 decreased (10.47 ± 0.91% vs. 27.86 ± ± 2.91%; P = 0.0015). The observed antiproliferative effect of RFNCS confirms the possibility and expedience of the development of complex pathogenetic therapy for malignant tumors of the brain using preparations obtained from fetal neurogenic cells.

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