The effect of iron dextran on the transcriptome of pig hippocampus

Abstract

The chain of events resulting from oxidative stress is still barely understood. The hippocampus is particularly vulnerable to oxidative stress. With the emergence of high-throughput sequencing, many different biological components can be discovered simultaneously. Using RNA-seq technology, we conducted a whole-transcriptome analysis of the hippocampi of piglets exposed to iron dextran (FeDex), a potent inducer of oxidation stress. The total hippocampal RNA from the piglets was sequenced, and 11.5 Gb of sequencing data were obtained. Regulatory molecules involved in oxidative stress were determined through the identification of 362 differentially expressed genes (DEGs). The functional analysis revealed that these DEGs were primarily involved in the defense response and responses to external stimuli. Furthermore, 76 neuropeptide gene transcripts and 60 neuropeptide receptor gene transcripts were identified in the hippocampus. Of these transcripts, the mRNA levels of six neuropeptide genes were significantly changed. Overall, this study describes the first use of RNA-seq to elucidate the transcriptomic changes in the piglet hippocampus caused directly by the injection of iron dextran, which enabled the characterization of the transcriptional response triggered by oxidative stress.

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