Cloning and analysis of promoter region of mouse immunoglobulin germline γ3 transcripts

Abstract

The germline γ3 transcripts (GLTγ3) is transcribed by mouse B cells following stimulation with lipopolysaccharide (LPS). GLTγ3 transcription is a prerequisite for IgG3 class switching. However, to date, the promoter activity of GLTγ3 has not been investigated. In this study, we constructed GLTγ3 promoter reporters and tested their promoter activities following LPS stimulation in B cells. Among the reporters, pGL3-γ3(F4) had the highest basal activity and was optimally activated by LPS. Deletion of the first and second conserved sequences (CS1 and CS2) in the GLTγ3 promoter abrogated LPS-induced promoter activity. In addition, DNase I-hypersensitive site 1,2 (HS1,2) further enhanced GLTγ3 promoter activity. These findings indicate that CS1 and CS2 of the GLTγ3 promoter are essential for LPS-induced GLTγ3 transcription and that HS1,2 enhances transcription.

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