Objective This study aimed to analyze cell viability and gene expression IL-6 (interleukin-6) and VEGF (vascular endothelial growth factor) after continuous ultrasound therapy of 1 and 3 MHz on L929 fibroblast cells. Design The L929 cells were cultivated in 12-well plates and divided into 5 groups: G1: non-irradiated, G2: 0.2 W/cm2-1 MHz, G3: 0.5 W/cm2-1 MHz, G4: 0.2 W/cm2-3 MHz and G5: 0.5 W/cm2-3 MHz. The cells were irradiated at 24 hours and 48 hours. Cell viability was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) method. The gene expression analysis was assessed using real-time PCR. Results The G2 and G3 groups showed a decrease in cell viability when compared to the G1 at 48 hours (p
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