Objective We examined the effects of twitch contraction induced by lumbar spinal root magnetic stimulation on immobilization-induced limited ROM and skeletal muscle fibrosis in rat soleus muscle. Design The groups included male Wistar rats (controls), rats with both bilateral ankle joints immobilized with plaster casts for 4 weeks (IM), and rats in which twitch contraction of the soleus muscle was induced by lumbar magnetic stimulation over the course of 4 weeks of immobilization (TC). Group differences in ankle dorsiflexion ROM, collagen content as determined by hydroxyproline assay, and the expression of fibrosis-related genes as determined by RT-PCR (hypoxia inducible factor-1α, α-smooth muscle actin, and types I and III collagen) in the soleus muscle were examined after the 4-week-long experiment. Results ROM in the TC group was significantly greater than that in the IM group. The hydroxyproline content and the expressions of fibrosis-related genes decreased significantly in the TC group compared to those in the IM group. No significant differences were seen in the expression of TGF-β mRNA. Conclusions These results suggest that twitch contraction induced by lumbar spinal root magnetic stimulation may reduce immobilization-induced limited ROM and skeletal muscle fibrosis. Corresponding author: Junya Sakamoto, Department of Physical Therapy, Unit of Physical and Occupational Therapy Sciences, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8520, Japan. TEL/Fax: +81-95-819-7964. E-mail: jun-saka@nagasaki-u.ac.jp Conflict of interest: None. Grant sponsor: This work was supported by a JSPS KAKEN Grant-in-Aid for Young Scientists (B) Number 24700539 from the Ministry of Education, Science, Sports and Culture (MEXT) Financial benefits to the authors: None Ethical approval: Animal care and experimental procedures were performed in accordance with the Guidelines for Animal Experimentation of Nagasaki University and were approved by the Institutional Animal Care and Use Committee. Copyright © 2018 Wolters Kluwer Health, Inc. All rights reserved.
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