Purpose Insufficient physical activity (PA) and inadequate sleep quality (SQ) and sleep duration (SD) are highly prevalent behaviours that increase the risk of developing many chronic health conditions. Evidence regarding bi-directional relationships between PA, SQ and SD is inconsistent. As changes in one of these behaviours may impact on the other, it is important to understand the nature of the bi-directional relationship between physical activity and sleep. Therefore, the aim of this study was to examine the relationship between changes in physical activity, sleep quality and sleep duration over a 2-year period in middle-aged adults. Methods Participants were adults aged 42-72 years from Brisbane, Australia. Mail surveys were used to assess PA, SQ, SD, socio-demographic and health characteristics in 2011 and 2013 (n=3,649). Multinomial logistic regression analyses were conducted to examine the relationships between patterns of change in PA, SQ and SD over the two years. Results Improving or maintaining good SQ was associated with increasing or maintaining PA and maintaining PA was associated with maintaining or increasing SQ (p
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