Genome-Wide Analysis of Multidrug and Toxic Compound Extrusion (MATE) Family in Diploid Cotton, G. raimondii and G. arboreum and Its Expression Analysis Under Salt, Cadmium and Drought Stress

The extrusion of toxins and substances at cellular level is a vital survival life process in plants under abiotic stress. The multidrug and toxic compound extrusion (MATE) gene family is largely involved in the exportation of toxins and other substrates. We undertook to carry out the genome-wide analysis of MATE gene families in Gossypium raimondii and Gossypium arboreum and assessed their expression levels under salt, cadmium and drought stresses. We identified 70 and 68 MATE genes in G. raimondii and G. arboreum respectively. Majority of the genes were predicted to be localized within the plasma membrane with a few being distributed in other cell parts. Based on phylogenetic analysis, the genes were subdivided into three subfamilies designated as M1, M2 and M3. Closely related members shared similar gene structures, thus were highly conserved in nature and found to have evolved majorly through purifying selection. The genes were distributed in all the chromosomes. Twenty-nine gene duplication events were detected, with segmental type being the dominant. GO annotation revealed the link to salt, drought and cadmium stresses. The genes exhibited differential expression, with GrMATE18, GrMATE34, GaMATE41 and GaMATE51 were significantly up regulated under drought, salt and cadmium stress, and possibly could be the candidate genes. The results of this study provide the very first information on the genome wide and functional characterization of MATE genes in diploid cotton. The results therefore would be important for breeders in the development of more stress tolerant cotton genotypes.

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