Παρασκευή 16 Φεβρουαρίου 2018

Exercise-induced Protein Arginine Methyltransferase Expression in Skeletal Muscle

imageABSTRACTPurposeThis study aimed to determine protein arginine methyltransferase 1 (PRMT1), -4 (also known as coactivator-associated arginine methyltransferase 1 [CARM1]), and -5 expression and function during acute, exercise-induced skeletal muscle remodeling in vivo.MethodsC57BL/6 mice were assigned to one of three experimental groups: sedentary, acute bout of exercise, or acute exercise followed by 3 h of recovery. Mice in the exercise groups performed a single bout of treadmill running at 15 m·min−1 for 90 min. Hindlimb muscles were collected, and quantitative real-time polymerase chain reaction and Western blotting were used to examine exercise-induced gene expression.ResultsThe PRMT gene expression and global enzyme activity were muscle-specific, generally being higher (P

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