AbstractPurposeCommonly used physical activity metrics tell us little about the intensity distribution across the activity profile. The purpose of this paper is to introduce a metric, the intensity gradient, which can be used in combination with average acceleration (overall activity level) to fully describe the activity profile.Methods1669 adolescent girls (sample 1) and 295 adults with type 2 diabetes (sample 2) wore a GENEActiv accelerometer on their non-dominant wrist for up to 7-days. Body mass index and percent body fat were assessed in both samples and physical function (grip strength, Short Physical Performance Battery, sit-to-stand repetitions) in sample 2. Physical activity metrics were: average acceleration (AccelAV); the intensity gradient (IntensityGRAD from the log-log regression line: 25 mg intensity bins (x)/time accumulated in each bin (y)); total moderate-to-vigorous physical activity (MVPA); and bouted MVPA (sample 2 only).ResultsCorrelations between AccelAV and the IntensityGRAD (r=0.39-0.51) were similar to correlations between AccelAV and bouted MVPA (r=0.48), and substantially lower than between AccelAV and total MVPA (r>0.93). The IntensityGRAD was negatively associated with body fatness in sample 1 (p0.93). The IntensityGRAD was negatively associated with body fatness in sample 1 (p
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