Oxytocin mediates the beneficial effects of the exercise training on breast cancer

Abstract

Exercise training can affect the breast tumor growth. We hypothesized that exercise training may reduce breast tumor growth by inducing Oxytocin (OT) secretion and its related signaling pathways such as PI3K/AKT and ERK. Therefore fifty-six BALB/c mice were equally divided into seven groups to study the effects of OT and atosiban (an oxytocin receptor antagonist) together with the interval exercise training on breast tumor growth, as well as tumor related signaling pathways including PI3K/AKT and ERK. Animal weight, OT plasma level, tumor weight and volume measured at the end of study. PI3K/AKT and ERK was evaluated by Western blot and qPCR assays.The results showed that OT plasma level was significantly increased in trained animals. The volume and weight of tumors were decreased significantly after both exercise training and OT administration. The expression of genes involved in the tumor cell proliferation such as PI3KR2, AKT and mTOR was noteworthy declined in the exercise training and OT-treated groups. Furthermore, the expression of genes involved in the cell apoptosis such as caspase 3 and Bax was significantly increased significantly in the tumor tissues. In addition, western blot results showed that the phosphorylated Akt and ERK were significantly decreased in the exercise training and OT groups compared to the tumor group. Interestingly, atosiban reversed these effects. These results indicated that the interval exercise training through OT secretion may reduce the PI3K/AKT and ERK axis activities, and consequently decrease the tumor volume and weight in a mice model of breast cancer.

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