Protein Digestion and Quality of Goat and Cow Milk Infant Formula and Human Milk Under Simulated Infant Conditions.

Objective: To determine the kinetics of true ileal protein digestion and digestible indispensable amino acid score (DIAAS) of a goat milk based infant formula (GIF), a cow milk based infant formula (CIF) and human milk (HM). Methods: The GIF, CIF and HM were investigated in an in vitro gastrointestinal model simulating infant conditions. Digested compounds were dialyzed from the intestinal compartment as bioaccessible fraction. Dialysate was collected in 15-60 min periods for 4 h. True ileal protein digestibility and DIAAS were determined as bioaccessible nitrogen (N) and amino acids (AA). Results: N bioaccessibility from the GIF showed similar kinetics to that of HM. The CIF showed a delay in N bioaccessibility vs. the GIF and HM. In the 1st hour of digestion, N bioaccessibility was 19.9 +/- 3.5% and 23.3 +/- 1.3% for the GIF and HM, resp., and 11.2 +/- 0.6% for CIF (P

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