Dissociating external power from intramuscular exercise intensity during intermittent bilateral knee-extension in humans

Abstract

Compared with work-matched high-intensity continuous exercise, intermittent exercise dissociates pulmonary oxygen uptake (V̇O₂) from the accumulated work. The extent to which this reflects differences in O₂ storage fluctuations and/or contributions from oxidative and substrate-level bioenergetics is unknown. Using pulmonary gas-exchange and intramuscular ³¹P magnetic resonance spectroscopy, we tested the hypotheses that at the same power: ATP synthesis rates are similar; but peak V̇O₂ amplitude is lower in intermittent vs. continuous exercise. Thus, we expected that: intermittent exercise relies less upon anaerobic glycolysis for ATP provision than continuous exercise; shorter intervals would require relatively greater fluctuations in intramuscular bioenergetics than in V̇O₂ compared with longer intervals. Six men performed bilateral knee-extensor exercise (estimated to require 110% peak aerobic power) continuously and with three different intermittent work:recovery durations (16:32; 32:64; 64:128 s). Target work duration (576 s) was achieved in all intermittent protocols; greater than continuous (252 ± 174 s; P < 0.05). Mean ATP turnover rate was not different between protocols (∼43 mm min⁻¹ on average). However, the intramuscular PCr component of ATP generation was greatest (∼30 mm min⁻¹), and oxidative (∼10 mm min⁻¹) and anaerobic glycolytic (∼1 mm min⁻¹) components lowest for 16:32 and 32:64 s intermittent protocols, compared with 64:128 s (18 ± 6, 21 ± 10 and 10 ± 4 mm·min⁻¹, respectively) and continuous protocols (8 ± 6, 20 ± 9 and 16 ± 14 mm min⁻¹, respectively). As intermittent work duration increased towards continuous, ATP production relied proportionally more upon anaerobic glycolysis and oxidative phosphorylation, and less upon PCr breakdown. However, performing the same high-intensity power intermittently vs. continuously reduced the amplitude of fluctuations in V̇O₂ and intramuscular metabolism, dissociating exercise intensity from the power output and work done.

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