Prognostic Significance of hMSH2, hMSH3 and hMSH6 Expression in Ameloblastoma

Publication date: Available online 7 June 2017
Source:Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
Author(s): Gleyson Kleber do Amaral-Silva, Celeste Sánchez-Romero, Vivian Petersen Wagner, Manoela Domingues Martins, Hélder Antônio Rebelo Pontes, Eduardo Rodrigues Fregnani, Fernando Augusto Soares, Oslei Paes de Almeida, André Caroli Rocha, Alan Roger Santos-Silva, Felipe Paiva Fonseca, Pablo Agustin Vargas
AimsTo investigate hMutS proteins in human dental germ, ameloblastomas and ameloblastic carcinoma, and to determine whether the expression of these proteins has any prognostic potential.Methods and resultsTen cases of human dental germs, 39 ameloblastomas and 2 ameloblastic carcinomas were used to determine the distribution of the proteins during the carcinogenesis process. Simultaneously, another sample of 73 ameloblastomas was arranged in tissue microarray and their clinical, microscopic and radiographic features, treatment outcome, presence of BRAF-V600E mutation and follow-up data, were assessed to determine the prognostic relevance of the proteins. Immunohistochemistry was performed using antibodies against hMutS (hMSH2, hMSH3, hMSH6) and Ki67. hMSH2 and hMSH6 were significantly down-expressed in ameloblastomas (p=0.0059) than in human dental germs (p<0.0001). hMSH2, hMSH3 and the protein combinations were significantly associated with BRAF-V600E mutation (p<0.05). Simultaneous over-expression of hMutS was associated with recurrences (p=0.035); however, the proteins did not predict the disease-free survival of patients (p>0.05).ConclusionshMutS proteins are down-regulated in ameloblastoma; moreover, simultaneous over-expression of these proteins in ameloblastoma was associated with recurrence, but did not predict disease-free survival.



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