High throughput sequencing of herbaceous peony small RNAs to screen thermo-tolerance related microRNAs

Abstract

Herbaceous peony (Paeonia lactiflora Pall.) is an ornamental flower with wide applications for landscape. However, its application potential is greatly restricted because it is prone to heat stress injury. Although there are some studies about thermo-tolerance of P. lactiflora which focused on physiological and transcriptional level, the information on post-transcriptional level is lacked. Here, we chose a thermo-tolerant cultivar 'Zifengyu' and a moderately thermo-tolerant cultivar 'Hongyanzhenghui' under the natural long summer heat to investigate heat responsive miRNAs by miRNA sequencing. A total of 24,008,974 and 21,981,360 raw reads were generated from 'Zifengyu' and 'Hongyanzhenghui', respectively, as well as 45,016,796 total sRNAs and 6,297,111 unique sRNAs were obtained. Then 271 conserved and 9 novel miRNAs were identified in 'Zifengyu', while 236 conserved and 11 novel miRNAs were identified in 'Hongyanzhenghui'. Among them, 71 known miRNAs with 272 potential target genes and 3 novel miRNAs with 4 potential target genes were significantly differentially expressed. Expression analysis of the 7 candidate miRNAs previously reported to be regulated by heat stress and their target genes were performed. It showed that miR172c-3p, miR395a, miR397a, miR408-5p and miR827 were up-regulated by heat stress, and expressed much higher in thermo-tolerant cultivar 'Zifengyu', suggesting that they might be involved in heat stress response at the post-transcriptional level. These results could provide a better understanding about the thermos-tolerant mechanism of P. lactiflora on post-transcriptional level.

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