Investigation of oxidative stress parameters in different life span erythrocyte fractions in young untrained men after an acute exercise

The objective of study was to establish the role of exercise-induced oxidative stress in the erythrocyte fractions: young (YF), middle-aged (MAF) and old (OF) of young untrained men after acute exercise. Blood samples were collected before exercise, immediately and one hour after exercise. Maximum wattage was 292±27 W and exercise time duration was 8.73±0.9 min. Different optical properties and oxidative stress parameters were found in each erythrocyte fraction. Total thiols in YF and MAF after exercise and after one hour rest were similar to values before exercise, however, in OF (32.7±9.8 nmol/mgHb) the concentration was lower in comparison to YF (55.5±3.2 nmol/mgHb), MAF (56.8±7.7 nmol/mgHb) and increased one hour later (P < 0.0002). The glutathione concentration was higher in OF (8.4±0.4 nmol/mgHb) than in YF (4.5±0.6 nmol/mgHb) and MAF (4.8±0.5 nmol/mgHb) (P < 0.0002) and did not change neither after exercise nor one hour later. In OF, the peroxides level was higher after exercise (1.2±0.2 nmol/mgHb) and one hour later (1.1±0.2 nmol/mgHb), when compared with samples before exercise (0.9±0.1 nmol/mgHb) (P < 0.05). Similar results were observed in YF and MAF. The TBARS (thiobarbituric acid reactive substances) level was approx. 2.5 fold higher in OF (0.19±0.04 nmol/mgHb) when compared with YF (0.07±0.01 nmol/mgHb) and MAF (0.08±0.02 nmol/mgHb) (P < 0.0002) and was increased after exercise remaining unchanged after one hour later. In YF and MAF, no difference in TBARS level was detected after exercise one hour later. No difference in membrane fluidity was observed in all fractions. Erythrocytes OF appeared to be more sensitive for cellular oxidative damages.

This article is protected by copyright. All rights reserved

from Physiology via xlomafota13 on Inoreader http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1113%2FEP085930
via IFTTT