Evaluation of analgesic effect of Gentamicin in thermally induced pain models in rats and mice

2016-10-05T07-48-38Z
Source: National Journal of Physiology, Pharmacy and Pharmacology
Raghunatha Rao Ponnaluri, Bhanu Prakash Kolasani, Raghunandan Mudium.
Background: Evidence has accumulated for the involvement of calcium ions in nociception and N-type voltage-dependent calcium channels being critical for pain transduction and modulation. N-type calcium channel blockers represent a new class of analgesics that are selective for calcium channels involved in pain signal transmission. Gentamicin, an aminoglycoside antibiotic was discovered to block these N-type voltage-dependent calcium channels. Aims and Objectives: The present study is to evaluate the analgesic activity of gentamicin in thermally induced pain models of rats and mice and compare it against the standard analgesic aspirin. Materials and methods: A total of 24 rats and 24 mice were distributed into four groups of 6 each: Group A received distilled water as control, Group B received Gentamicin- low dose (80 μg/kg), Group C received Gentamicin- high dose (160 μg/kg), and Group D received standard drug Aspirin (20 mg/kg in rats and 25 mg/kg in mice); all drugs were given intraperitoneally. Analgesic activity was determined using tail flick method and hot plate method. In both the methods, the mean reaction time (MRT) in seconds at 0, 30, 60, 90, and 120 min among the four groups were noted in both rats and mice. The percentage increase in MRT was calculated which indicates the degree of analgesia produced. Results: In the tail flick test, increase in the MRT was statistically significant (P

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