IN VITRO ANTI - CLASTOGENIC ACTIVITIES OF DIFFERENT FRACTIONS OF ROOT OF LAWSONIA INERMIS ON CALF THYMUS DNA MOLECULE BY GEL ELECTROPHORESIS

2016-08-09T05-51-53Z
Source: Indo American Journal of Pharmaceutical Research
MinaBasirian*, V. Ch. Jeevani, S. N. Manjula, K. Mruthunjaya.
Back ground: Most of the mutations are due to clastogens, hence finding the potent anti-oxidants which inhibit the mutation is very important. In vitro anti-clastogenic activity of different fractions (extraction) of roots of Lawsonia inermis (LI), on calf thymus DNA, against cigarette smoke, CYCLO (cyclophosphamide) and hydroxyl radical as clastogens, were studied. These clastogens lead to breakdown of the DNA molecule and mutation, hence present study focused on effect different fractions of LI which are having anti-oxidants property to prevent DNA strand break. Methods: Anti-clastogenic activity of different fractions of LI namely ethyle acetate (LIEAC), alcoholic (LIALC), petroleum ether (LIPET), buthanol (LIBUT) and aqueous (LIAQ) were evaluated on calf thymus DNA by agarose gel electrophoresis against CYCLO (cyclophosphamide), cigarette smoke and hydroxyl radical (Fenton reaction) as clastogens. DNA strand after exposure to different clastogens were breaking, based on different clastogens , formation of different sized breakage of DNA were observed on UV illumination with help of dye. Here the clastogens were breaking the DNA stand and prevention of the breaking down was observed for different fractions of LI. Results: Protection (inhibit the breaking of DNA strand) of Calf thymus DNA against Fenton reaction, CYCLO, cigarette, smoke induced damage was observed in all the fractions of LI due to their anti-oxidant activity, but LIEAC , LIALC and LIBUT showed more protective activity ,and reduced the DNA strand break Conclusion: LI fractions showed protective and anti-clastogenic activity against clastogens which induced mutation. The protective activity of different fraction was due to anti-oxidant property which is directly related to total phenolic and flavonoid content but still further investigation required to claim the protective activity of these fractions.


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