HPLC SEPARATION OF PIDOTIMOD ENANTIOMERS USING BETA-CYCLODEXTRIN BASED CHIRAL STATIONARY PHASE

2016-08-09T05-51-53Z
Source: Indo American Journal of Pharmaceutical Research
Madhuri Baghel, Sadhana J. Rajput*.
A simple, sensitive, specific, precise, and rapid isocratic chiral RP-HPLC method was developed and validated for estimation of R and S enantiomers of Pidotimod, a potent dipeptide immunomodulator. The separation was achieved with LICHROCART CHIRADEX (150×4.6mm, 5μm) column at wavelength of 215 nm. The mobile phase was ammonium acetate buffer and methanol in the ration of 85:15 at flow rate of 0.8 ml/ min. The developed method was validated as per ICH guideline which was linear over the range of 50-250 μg/ml with LOD and LOQ of 1.2072 and 3.6583 respectively. The %RSD for repeatability and Intermediate precision was found to be less than 2. The elution of enantiomeric peaks of Pidotimod was further confirmed by LC-MS/MS analysis. The developed method could be utilized for estimation of pidotimod enantiomers.


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