Τετάρτη 20 Απριλίου 2016

Sex Differences in Drosophila Somatic Gene Expression: Variation and Regulation by Doublesex

Sex differences in gene expression have been widely studied in Drosophila melanogaster. Sex differences vary across strains, but many molecular studies focus on only a single strain or on genes that show sexually dimorphic expression in many strains. How extensive variability is and whether this variability occurs among genes regulated by sex determination hierarchy terminal transcription factors is unknown. To address these questions, we examine differences in sexually dimorphic gene expression between two strains in Drosophila adult head tissues. We also examine gene expression in doublesex (dsx) mutant strains to determine which sex-differentially expressed genes are regulated by DSX and the mode by which DSX regulates expression. We find substantial variation in sex-differential expression. The sets of genes with sexually dimorphic expression in each strain show little overlap. The prevalence of different DSX regulatory modes also varies between the two strains. Neither the patterns of DSX DNA occupancy, nor mode of DSX regulation explain why some genes show consistent sex-differential expression across strains. We find that the genes identified as regulated by DSX in this study are enriched with known sites of DSX DNA occupancy. Finally, we find that sex-differentially expressed genes and genes regulated by DSX are highly enriched on the fourth chromosome. These results provide insights into a more complete pool of potential DSX targets, as well as reveal the molecular flexibility of DSX regulation.



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Binding Sites in the EFG1 Promoter for Transcription Factors in a Proposed Regulatory Network: A Functional Analysis in the White and Opaque Phases of Candida albicans

In Candida albicans the transcription factor Efg1, which is differentially expressed in the white phase of the white-opaque transition, is essential for expression of the white phenotype. It is one of six transcription factors included in a proposed interactive transcription network regulating white-opaque switching and maintenance of the alternative phenotypes. Ten sites were identified in the EFG1 promoter that differentially bind one or more of the network transcription factors in the white and/or opaque phase. To explore the functionality of these binding sites in the differential expression of EFG1 expression, we generated targeted deletions of each of the ten binding sites, combinatorial deletions and regional deletions, using a Renilla luciferase reporter system. Individually targeted deletion of only four of the 10 sites had minor affects consistent with differential expression of EFG1, and only in the opaque phase. Alternative explanations are considered.



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Fine Mapping of the Barley Chromosome 6H Net Form Net Blotch Susceptibility Locus

Net form net blotch caused by the necrotrophic fungal pathogen Pyrenophora teres f. teres is a destructive foliar disease of barley with the potential to cause significant yield loss in major production regions throughout the world. The complexity of the host-parasite genetic interactions in this pathosystem hinders the deployment of effective resistance in barley cultivars, warranting a deeper understanding of the interactions. Here we report on the high-resolution mapping of the dominant susceptibility locus near the centromere of chromosome 6H in the barley cultivars Rika and Kombar that are putatively targeted by necrotrophic effectors from P. teres f. teres isolates 6A and 15A, respectively. Utilization of progeny isolates derived from a cross of P. teres f. teres isolates 6A x 15A harboring single major virulence loci (VK1, VK2 and VR2) allowed for the Mendelization of single inverse gene-for-gene interactions in a high-resolution population consisting of 2976 Rika x Kombar recombinant gametes. Brachypodium distachyon synteny was exploited to develop and saturate the susceptibility region with markers, delimiting it to ~0.24 cM and a partial physical map was constructed. This genetic and physical characterization further resolved the dominant susceptibility locus, designated Spt1 (susceptibility to P. teres f. teres). The high-resolution mapping and cosegregation of the Spt1.R and Spt1.K gene/s indicates tightly linked genes in repulsion or alleles possibly targeted by different necrotrophic effectors. Newly developed barley genomic resources greatly enhance the efficiency of positional cloning efforts in barley as demonstrated by the Spt1 fine mapping and physical contig identification reported here.



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Genome Sequence and Analysis of a Stress-Tolerant Wild-Derived Strain of Saccharomyces cerevisiae used in Biofuels Research

The genome sequences of more than 100 strains of the yeast Saccharomyces cerevisiae have been published. Unfortunately, most of these genome assemblies contain dozens to hundreds of gaps at repetitive sequences, including transposable elements, tRNAs, and subtelomeric regions, which is where novel genes generally reside. Relatively few strains have been chosen for genome sequencing based on their biofuel production potential, leaving an additional knowledge gap. Here we describe the nearly complete genome sequence of GLBRCY22-3 (Y22-3), a strain of S. cerevisiae derived from the stress-tolerant wild strain NRRL YB-210 and subsequently engineered for xylose metabolism. After benchmarking several genome assembly approaches, we developed a pipeline to integrate Pacific Biosciences (PacBio) and Illumina sequencing data and achieved one of the highest quality genome assemblies for any S. cerevisiae strain. Specifically, the contig N50 is 693 kbp, and the sequences of most chromosomes, the mitochondrial genome, and 2-micron plasmid are complete. Our annotation predicts 92 genes that are not present in the reference genome of the laboratory strain S288c, over 70% of which were expressed. We predicted functions for 43 of these genes, 28 of which were previously uncharacterized and unnamed. Remarkably, many of these genes are predicted to be involved in stress tolerance and carbon metabolism and are shared with a Brazilian bioethanol production strain, even though the strains differ dramatically at most genetic loci. The Y22-3 genome sequence provides an exceptionally high-quality resource for basic and applied research in bioenergy and genetics.



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An Improved Genome Assembly of Azadirachta indica A. Juss.

Neem (Azadirachta indica A. Juss.), an evergreen tree of the Meliaceae family, is known for its medicinal, cosmetic, pesticidal and insecticidal properties. We had previously sequenced and published the draft genome of the plant, using mainly short read sequencing data. In this report, we present an improved genome assembly generated using additional short reads from Illumina and long reads from Pacific Biosciences SMRT sequencer. We assembled short reads and error corrected long reads using Platanus, an assembler designed to perform well for heterozygous genomes. The updated genome assembly (v2.0) yielded 3- and 3.5-fold increase in N50 and N75, respectively; 2.6-fold decrease in the total number of scaffolds; 1.25-fold increase in the number of valid transcriptome alignments; 13.4-fold less mis-assembly and 1.85-fold increase in the percentage repeat, over the earlier assembly (v1.0). The current assembly also maps better to the genes known to be involved in the terpenoid biosynthesis pathway. Together, the data represents an improved assembly of the A. indica genome. The raw data described in this manuscript are submitted to the NCBI Short Read Archive under the accession numbers SRX1074131, SRX1074132, SRX1074133, and SRX1074134 (SRP013453).



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Genetic Interactions Between the Meiosis-Specific Cohesin Components STAG3 REC8 and RAD21L

Cohesin is an essential structural component of chromosomes that ensures accurate chromosome segregation during mitosis and meiosis. Previous studies have shown that there are cohesin complexes specific to meiosis, required to mediate homologous chromosome pairing, synapsis, recombination and segregation. Meiosis-specific cohesin complexes consist of two structural maintenance of chromosomes proteins (SMC1α/SMC1β and SMC3), an α-kleisin protein (RAD21, RAD21L or REC8), and a stromal antigen protein (STAG1, 2 or 3). STAG3 is exclusively expressed during meiosis, and is the predominant STAG protein component of cohesin complexes in primary spermatocytes from mouse, interacting directly with each α-kleisin subunit. REC8 and RAD21L are also meiosis-specific cohesin components. Stag3 mutant mice arrest in early prophase ("zygotene-like"stage), displaying failed homolog synapsis and persistent DNA damage, as a result of unstable loading of cohesin onto the chromosome axes. Interestingly, Rec8, Rad21L double mutants resulted in an earlier "leptotene-like" arrest accompanied by complete absence of STAG3 loading. To assess genetic interactions between STAG3 and α-kleisin subunits RAD21L and REC8, our lab generated Stag3, Rad21L and Stag3, Rec8 double knockout mice, and compared them to the Rec8, Rad21L double mutant. These double mutants are phenotypically distinct from one another and more severe than each single knockout mutant with regards to chromosome axis formation, cohesin loading, and sister chromatid cohesion. The Stag3, Rad21L and Stag3, Rec8 double mutants both progress further into prophase I than the Rec8, Rad21L double mutant. Our genetic analysis demonstrates that cohesins containing STAG3 and REC8 are the main complex required for centromeric cohesion, and RAD21L cohesins are required for normal clustering of pericentromeric heterochromatin. Furthermore, the STAG3/REC8 and STAG3/RAD21L cohesins are the primary cohesins required for axis formation.



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Infection with fully mature Corynosoma cf. validum causes ulcers in the human small intestine

Abstract

Corynosoma is a parasite that can normally be found in the intestinal tract of fish-eating mammals, particularly in seals and birds. The present case proposed that Corynosoma could attain full maturity in the human intestine. A 70-year-old female complained of abdominal pain. A computed tomography (CT) scan revealed a swelling of the intraperitoneal lymph nodes with no responsible lesion. Video capsule endoscopy and double-balloon endoscopy detected several ulcerations and one parasite in the ileum, which was tightly attached at the bottom of the ulcerations. The parasite was cylindrical and measured approximately 10 mm (long) x 3 mm (wide). Pathologically, the worm had a four-layered body wall and contained embryonated eggs. The sequences of the parasite-derived nuclear ribosomal DNA fragment and mitochondrial DNA fragment of cox1 were almost identical to those of Corynosoma validum. The patient's abdominal pain immediately improved after the administration of pyrantel pamoate (1,500 mg). Corynosoma was possibly the responsible disease in a patient who complained of abdominal pain and in whom no responsible lesion was detected by CT, gastroduodenoscopy or colonoscopy. Examinations of the small intestines should be aggressively performed in such cases.



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Blauer Announces New ArmorSkin Polo Casual Comfort with a Professional Appearance

Boston, Massachusetts — Blauer is proud to announce the creation of a highly-demanded addition to its popular ArmorSkin® line: the ArmorSkin Polo. By combining the best in materials technology with the casual styling used as the uniform of the day for many in public safety, the ArmorSkin Polo allows for ultimate breathability and heat release for details, traffic, bike patrol, Fire and EMS ...

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Emergence and Dissemination of ST131 Escherichia coli Isolates Among Patients with Hospital-Acquired Pneumonia in Asian Countries

Microbial Drug Resistance , Vol. 0, No. 0.


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Editorial Board

Publication date: March 2016
Source:Arab Journal of Gastroenterology, Volume 17, Issue 1





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Photo of EMT mowing patient's lawn goes viral

SACRAMENTO, Calif. — A photo of an EMT mowing a lawn after an elderly man passed out has gone viral.

Ken Densley said his 87-year-old father, who refuses to let anyone else mow his lawn, was working on his yard when he pushed too hard and passed out Saturday. Neighbors saw what happened and called 911.

Densley said as they were taking his dad back inside, he noticed something else.

"One of the EMTs put the gurney away and the other finished mowing the lawn," he said. "He finished and even put the mower away."

Densley took a picture of the EMT and shared it on Facebook. The photo has garnered more than 10,000 likes and almost 2,000 shares.

Densley said his father was discharged from the hospital on Sunday and is doing great.



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Epithelial Cell Extrusion Zones Observed on Confocal Laser Endomicroscopy Correlates with Immunohistochemical Staining of Mucosal Biopsy Samples

Abstract

Background and Aims

The density of epithelial cell extrusion zones in the intestinal lining, also known as gap density (number of gaps/1000 epithelial cells counted), can be quantitated using probe-based confocal laser endomicroscopy (pCLE). Gap density has been reported to be higher than normal in both inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) patients. Epithelial cells destined for extrusion from the intestinal surface would stain positive for either activated caspase-1 or caspase-3 on mucosal biopsy samples. The aim of this study was to determine whether epithelial gap density on pCLE correlates with quantitative analysis of activated caspase staining of mucosal biopsy samples from patients.

Methods

We obtained pCLE images and biopsy samples of the terminal ileum during colonoscopies of healthy controls and patients with either IBD or IBS. The pCLE images and biopsy samples were blindly analyzed for gap density and for cells staining positive for activated caspases, respectively. The degree of correlation was determined using nonparametric statistical tests.

Results

The median results were 10 gaps/1000 cells counted for controls versus 33 gaps/1000 cells counted for chronic intestinal disorder patients (p = 0.02). Activated caspase staining showed 13 positive cells/1000 epithelial cells counted versus 26 positive cells/1000 epithelial cells counted, respectively (p = 0.02), thus showing a strong correlation with a Spearman's coefficient ρ of 0.61 (strong correlation for ρ = 0.4–0.75, p = 0.01).

Conclusions

Intestinal epithelial gap density via pCLE correlated strongly with quantitative analysis of immunohistochemical staining of mucosal biopsy samples.



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Rescue task forces and the scene safety dilemma

We can save more lives in large scale active shooter situations, but it will require us to rethink everything we've been teaching EMS providers about scene safety.

On April 20, 1999, I sat in my ambulance and listened to the radio while Dave Sanders slowly bled to death a few hundred yards away.

It wasn't an oversight.

We knew he was there. Inside Columbine High School, a fellow teacher had scrawled the message "1 bleeding to death" on a piece of paper and placed it in a window.

Police were aware of Sander's location and his situation. Inside the classroom a small vigil of students, the same students Sanders helped to protect, gathered around him and showed him pictures of his three children while he slowly faded from consciousness.

As the note in the window predicted, the 47-year-old computer science teacher did bleed to death. On the day of the Columbine Massacre, we failed Dave Sanders. For that reason, I'll never be able to forget him.

Lessons of Columbine
Fortunately, a lot has changed in law enforcement since 1999. The lessons of Columbine spurred the creation of law enforcement rapid intervention teams and policies that allow officers to quickly engage violent attackers. Their success has been instrumental in stopping many of these incidents in minutes, instead of the hours that passed at Columbine.

Part of doing this work is analyzing our failures and finding the keys to better performance in the future. When it comes to active shooter events, law enforcement is now 20 years ahead of EMS. The evolution of law enforcement has been tremendous. However, if it happened again today, most systems across the United States would consign me to the same role I played almost 20 years ago, sitting idle in my medic unit and waiting for someone to declare the shooter or shooters dead and the scene clear.

When articles appear promoting the idea of an EMS rescue task force, to enter these scenes and help extract wounded victims during the incident, while police are still engaging the shooter, the ideas are met with a predictable barrage of scene safety advocates calling the idea ludicrous and reckless.

Reconsidering 'scene safety'
I understand. We''e been teaching scene safety as an absolute for so long, why wouldn't the EMS safety establishment rise up and declare the idea heresy" Proposing that EMS enter a scene when safety is not an iron clad guarantee flies in the face of everything we've been teaching for darn near half a century. Which is perhaps the best reason to consider it.

Every other public safety discipline has had to embrace the muddy waters of risk-benefit analysis, deciding when the risk is acceptable for the potential lifesaving reward, while EMS has remained clean by dodging the issue entirely. By teaching an absolutist view of scene safety, we dodge the complexities of the proverbial scene safety bullet.

In teaching that we do not enter the scene unless it is completely safe, we offer an oversimplified version of a more complex truth. I've also come to believe that we do our people a disservice. The idea that the scene can be made safe is a myth. And we'd do better to recognize the more complex and uncomfortable truth.

You do a dangerous job
A lot of the pushback from the scene safety absolutists is push back about being forced to do what is perceived as dangerous work. "If you want to do dangerous things," they argue, "go be a cop or a firefighter."

Another observes that, "Nothing matters more than going home to my family at the end of my shift."

I agree that going home to your family is of paramount importance. If it is your first consideration when making life choices, might I suggest that you already signed up for a deceptively dangerous job. Driving a vehicle emergent — red lights and sirens — is dangerous. Working on a freeway at night is remarkably dangerous. Drunk people can be dangerous. Entering homes with little idea of what's happening inside is dangerous.

If you feel like your job is safe and the rescue task force folks are suggesting a much more dangerous version of your job, I'd like to suggest that you're being naive about the dangers of your job. And I genuinely believe that you will be safer by overcoming your naiveté.

Safety is not absolute, it's relative
I cringe when I hear people say, "I don't enter the scene until the scene is safe."

Your safety isn't a light switch that can be turned off and on. If you believe it is, may I offer you a 9mm lesson in scene safety"

The scene is never safe. Even the most benign looking calls can rapidly deteriorate and most of the folks who are injured or die in the line of duty have this in common … they never saw it coming.

When you change your mindset to recognize the complex relative nature of your personal safety, you'll start evaluating your safety on an ongoing basis. When you consider that your initial assessment of the safety of the scene may be incorrect, you'll become more diligent and aware.

You'll also start actively considering the current risk profile of your environment and doing a true risk benefit analysis. You start asking yourself, are the risks currently being incurred by my team worth the benefit of our presence" When you recognize the relative nature of safety, you become safer.

You are already doing risk-benefit analysis
Having the ability to deploy medics into a scene to extract victims during an active killer incident doesn't mean that it becomes the automatic response to the scenario. It will be appropriate in some cases and not in others.

This requires us to think our way through the scene and understand the capabilities and weaknesses of our team. It means we will need to evaluate the threat, consider the unknowns and evaluate the possible gains.

You already do this. When you decide whether or not a drunk or uncooperative patient will be placed in restraints, you do risk-benefit analysis. You also do it every time you decide to turn your back on a patient to grab a blood pressure cuff.

If you sometimes drive a little bit faster en route to a child not breathing or step aside and allow a police officer to go through a door in front of you instead of behind you, you understand how we sometimes assume additional risk or choose a more conservative path.

You're likely doing this to some degree on every call you run. You risk more to save more. You risk less to save less. You decide how fast you drive. You decide what environment you're comfortable entering. You decide when to stay and when to go.

Risk-benefit analysis isn't recklessness
Some folks will tell you that EMS personnel who want to enter a shooting or stabbing scene behind police officers are reckless cowboys looking for a thrill. On the contrary, I think the people advocating for this capability are some of the most risk conscious among us.

When we are risk conscious we see that our risk tolerance can adapt to the needs of the scene. When a car accident in the middle of the freeway appears minor we may wait in our vehicle for additional resources to control traffic. When the car is overturned and people are lying in the road, we may choose to use our vehicle to shield them and attempt to rapidly remove them.

The scene has the same risk profile. But the circumstances warrant a different — perhaps more risky — approach. This isn't reckless, this is a willingness to apply our judgement and decision making capacity to the problem as it presents itself to us in real time. A willingness to work with less than ideal certainty, when the potential for benefit is great, isn't reckless, it's thoughtful, rational and reasonable.

Let's get real about the dangers of EMS
I hope the rescue task force idea takes hold. It could do a lot of good for folks injured inside hostile environments. The next Dave Sanders might be someone you love. Beyond the benefit to this small group of potential patients, it could also do us a lot of good as an industry, by compelling us to stop preaching the easy-road on scene safety.

It could finally force us to recognize that safety is not an absolute, to teach that reasoned risk-benefit analysis is essential and to get real with each other about the dangers of working in emergency services.



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Ultrasound pattern sum score and regional nerve enlargement index for differentiation of demyelinating inflammatory and hereditary neuropathies

Inherited neuropathies generally present with distally accentuated paresis of the legs with predominance of the peroneal muscles, osseous anomalies (e.g. pes cavus, scoliosis), sensory deficits, missing or weak deep tendon reflexes. Positive family history encourages the diagnosis (Patzko et al., 2012). It may be confirmed by nerve conduction studies (NCS, e.g. axonal or demyelinating predominance). Finally, molecular analysis corroborates the diagnosis, e.g. by finding PMP 22 pathology. The autosomal-dominant Charcot-Marie-Tooth (CMT) 1a type (PMP22 duplication) is the most frequent demyelinating type, while CMT1b (MPZ mutation) is rarer.

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Effect of fampridine on axonal excitability in multiple sclerosis

Multiple sclerosis (MS) is an inflammatory demyelinating and neurodegenerative disorder of the human central nervous system and is the most common cause of non-traumatic chronic neurological disability among the young adult population (Hauser et al., 2006, Dutta et al., 2011). Eighty-five percent of new-onset MS is typically characterized by a relapsing-remitting (RRMS) course (Ransohoff et al., 2015). With the advent of novel disease modifying drugs that effectively expands the neurologist's therapeutic armamentarium, the frequency of clinical and/or radiological relapses are reduced.

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Single electroencephalographic patterns as specific and time-dependent indicators of good and poor outcome after cardiac arrest

For many decades EEG has been used routinely for prognostication in postanoxic comatose patients, using various classifications before therapeutic hypothermia (TH) entered clinical practice(e.g. Hockaday et al., 1965; Synek 1988; Young et al., 1997). Despite this widespread use of EEG in clinical practice, the guidelines for prognostication in postanoxic patients published by the American Academy of Neurology (Wijdicks et al., 2006) did not recommend the use of EEG as its prognostic accuracy was still considered insufficient on the basis of pre-TH evidence of EEG studies.

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Characteristics of local field potentials correlate with pain relief by deep brain stimulation

Neuropathic pain is one of the most debilitating diseases and it usually responds poorly to conventional pharmacological treatment (Dworkin et al., 2007; Dworkin et al., 2010; Smith, 2012). In recent years, deep brain stimulation (DBS) of the sensory thalamus, periventricular gray / periaquedauctal gray (PVAG) and anterior cingulate cortex have been used to treat neuropathic pain (Hosobuchi et al., 1973; Mazars, 1975; Bittar et al., 2005; Owen et al., 2006a, Boccard et al., 2014, Russo et al., 2015).

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Who wins when paramedics fight BLS use of an ALS intervention?

Alright, let's circle around the campfire and let me tell you a story about EMS history. It starts like this:

Once upon a time …

There was a mythical creature called Paragod. You could recognize the creature in a flash. Paragod wore a utility belt loaded with bandage shears, a window punch and forceps. Paragod carried a 65-pound lightning generator in one hand and in the other an oversized orange and white tackle box filled with magic potions that revived the dead. Paragod used special tools to breathe air into lungs and pour special liquids into veins that gave life.

All of these gifts were fiercely protected by the Paragods. No mere mortals could use them in the field as they would be too dangerous in the wrong hands. Paragods objected to attempts by the Firemenicus or Ee-Em-Tee tribes to acquire and use those gifts.

But times were about to change.

Do you recognize a few parallels in this parable"

The fact is the line between so-called "basic" and "advanced" life support has been blurring for quite some time. Think of esophageal obturators in the 1970s, AEDs in the 1980s, epinephrine intramuscular injections in the 1990s and CPAP in the 2000s.

Today, there are states that authorize their EMTs to perform interventions formerly restricted to the ALS practice. While some of the procedures are dubious in their efficacy, others are clearly time-dependent and can reverse the life-threatening situation quickly.

The use of naloxone by laypersons, law enforcement and first responders is a contemporary example. As with any epidemic, public health procedures must be quickly developed and implemented to stem the threat.

Clearly there are some risks associated with injection, and training is required, along with a comprehensive monitoring and analysis program. As this report about Canadian firefighters being trained on the delivery of naloxone to opioid overdose patients points out, there is an urgency to reverse the effect of respiratory depression quickly.

While some may argue that assisting ventilations with a bag mask may be adequate, that simply is supportive care, not a reversal. On the other hand, the chance of taking the brake off of a polydrug overdose is very real, resulting in an agitated and potentially increases the deleterious effects of the remaining stimulant. However, I do believe that the benefit outweighs the risk in the majority of situations; good history taking, a careful physical exam and judicious administration can minimize the harm in more risky incidents.

There must also be a way to restrict the rising cost of what is a cheap drug — naloxone — to manufacture and package.

By the way, there is another Paragod story for our next campfire. In that story, the Almightynurses feel threatened by the rise of mobile integrated health initiatives.



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The quantified EEG characteristics of Responders and Non-Responders to long-term treatment with Atomoxetine in children with Attention Deficit Hyperactive Disorders

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Publication date: Available online 20 April 2016
Source:International Journal of Psychophysiology
Author(s): Giuseppe Augusto Chiarenza, Robert Chabot, Robert Isenhart, Luciano Montaldi, Marco Paolo Chiarenza, Maria Grazia Lo Torto, Leslie S. Prichep
Objective.The aim of our study is to examine quantitative Electroencephalogram (QEEG) differences between ADHD patients that are Responders and Non-Responders to long-term treatment with Atomoxetine at baseline and after 6 and 12months of treatment. Patients with Attention Deficit Hyperactivity Disorder (ADHD) received atomoxetine titrated, over 7days, from 0.5 to 1.2mg/kg/day. QEEG and Swanson, Nolan, and Pelham–IV Questionnaire (SNAP-IV) scores were recorded before treatment and after therapy.MethodsTwenty minutes of eyes closed resting EEG was recorded from 19 electrodes referenced to linked earlobes. Full frequency and narrow band spectra of two minutes of artifact-free EEG were computed as well as source localization using Variable Resolution Electrical Tomography (VARETA). Abnormalities were identified using Z-spectra relative to normative values.ResultsPatients were classified as responders, non-responders and partial responders based upon the SNAP-IV findings. At baseline, the responders showed increased absolute power in alpha and delta in frontal and temporal regions, whereas, non responders showed increased absolute power in all frequency bands that was widely distributed. With treatment responders' absolute power values moved toward normal values, whereas, non responders remained at baseline values.ConclusionsPatients with increased power in the alpha band with no evidence of alterations in the beta or theta range, might be responders to treatment with atomoxetine. Increased power in the beta band coupled with increased alpha seems to be related to non-responders and one should consider atomoxetine withdrawal, especially if there is persistence of increased alpha and beta accompanied by an increase of theta.



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No call no show no thank you: EMS agency cracks down on flaky employees

CHARLOTTE HALL, Md. — EMS job seekers are given specific reasons to not apply for a position in a job posting for paramedics, EMTs and dispatchers.

The Patriot Medical Transport System classified advertisement, posted April 4 to Southern Maryland Online, explains that EMS is an out of the ordinary workplace and 24/7 position.

Applicants who are expecting to sleep at work, lack reliable childcare or transportation, or need a flexible schedule are cautioned about applying.

"All are inspired by things we have been told by employees," said Vic DeMattia, owner of Patriot Medical Transport. "There is a disconnect between applicants saying they are available in an interview and then not being able to do the job. At the start of the job interview I always tell people one of the hardest things about the job is showing up for work."

The full posting reads:

"DON'T APPLY IF:
You do not understand that we work 24/7, you want a 9-5 job, you expect to get off on time every day, you can't make it to work on time, you need a ride to work and expect us to change your schedule, you need your cell phone connected to facebook, you can't talk and work at the same time, you have a flat tire or car accident every week and can't make it to work, you call out sick every week, you take your problems out on other people, another part time job will interfere with employment here, you have not arraigned (sic) for child care, you expect to sleep while on the clock, you think your volunteer work pays your bills, after 2 days of training you know everything, or all other EMS providers are stupid."

This is the first time Patriot has used what DeMattia describes as a "tongue in cheek" ad which also points to serious issues in finding and retaining qualified employees.

"The volume of applicants is less," DeMattia said. "But our current employees are getting more questions about working here which is leading to more referrals."

A shortage of good applicants is driving up the starting salary for EMTs and paramedics in Maryland. "We have lots of competition and a shortage of good people," DeMattia said.

DeMattia described the cost, upwards of $2,000, and time spent on hiring and training a new employee as increasingly difficult. "It is disheartening to spend all that time to end up with performance and moral issues," Demattia said.

For some EMS providers working at Patriot, like other private ambulance services, is the first step in a medical or public safety career. "We hire lots of young medics who use this as a stepping stone," DeMattia said.

Patriot Medical Transport provides interfacility transport and other types of non-emergency and non-911 patient transfers in a three county area of rural Maryland.



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Sonographic Appearance of the Tensor of the Vastus Intermedius



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Effect of Hot Vapor Smoothing Process on Surface Hardness of Fused Deposition Modeling Parts

3D Printing and Additive Manufacturing , Vol. 0, No. 0.


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Is applanation tonometry a reliable method for monitoring blood pressure in morbidly obese patients undergoing bariatric surgery?

Background

The aim of this study was to evaluate the validity of non-invasive continuous BP measurement by applanation tonometry in morbidly obese patients undergoing bariatric surgery.

Methods

Arterial blood pressure (AP) was recorded intraoperatively both by applanation tonometry (AT) (T-Line 200pro, Tensys Medical®, USA) and an arterial line (AL) after radial cannulation in obese patients undergoing bariatric surgery. Discrepancies between the two methods were assessed as bias, limits of agreement and percentage error. Mean, systolic, and diastolic arterial pressures were assessed (MAP, SAP, DAP respectively). Trending ability was assessed by concordance based on four-quadrant plotting.

Results

Mean (sd) BMI of the 28 patients was 49.4 (9.7 kg m–2). A total of 201 907 time points were available for analysis. Bias for MAPAT compared with MAPAL was +3.97 mm Hg (SAPAT +3.45 mm Hg; DAPAT +3.66 mm Hg) with limits of agreement for MAPAT of –14.47 and +22.41 mm Hg (SAPAT –22.0 and +28.9 mm Hg; DAPAT –15.7 and +23.1 mm Hg). Percentage error for MAPAT was 23.5% (23.4% for SAPAT; 30.5% for DAPAT). Trending ability for MAP, SAP, and DAP revealed a concordance of 0.74, 0.72, and 0.71, respectively.

Conclusions

Continuous BP assessment by applanation tonometry is feasible in morbidly obese patients undergoing bariatric surgery. However, despite a low mean difference, 95% limits of agreement and trending ability indicate that the technology needs to be improved further, before being recommended for routine use in this group of patients.



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The quality of turning in Parkinsons disease: a compensatory strategy to prevent postural instability?

The ability to turn while walking is essential for daily living activities. Turning is slower and more steps are required to complete a turn in people with Parkinson's disease (PD) compared to control subjects...

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Design and Development of Scaffolds for Tissue Engineering Using Three-Dimensional Printing for Bio-Based Applications

3D Printing and Additive Manufacturing , Vol. 0, No. 0.


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Paediatric difficult airway management: what every anaesthetist should know!



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A novel accelerometry-based algorithm for the detection of step durations over short episodes of gait in healthy elderly

The assessment of short episodes of gait is clinically relevant and easily implemented, especially given limited space and time requirements. BFS (body-fixed-sensors) are small, lightweight and easy to wear se...

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Seeing is believing: getting the best out of videolaryngoscopy



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Evaluation of distribution coefficients and concentration ratios of 90Sr and 137Cs in the Techa River and the Miass River

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Publication date: July 2016
Source:Journal of Environmental Radioactivity, Volumes 158–159
Author(s): E.A. Shishkina, E.A. Pryakhin, I.Ya Popova, D.I. Osipov, Yu Tikhova, S.S. Andreyev, I.A. Shaposhnikova, E.A. Egoreichenkov, E.V. Styazhkina, L.V. Deryabina, G.A. Tryapitsina, V. Melnikov, G. Rudolfsen, H.-C. Teien, M.K. Sneve, A.V. Akleyev
Empirical data on the behavior of radionuclides in aquatic ecosystems are needed for radioecological modeling, which is commonly used for predicting transfer of radionuclides, estimating doses, and assessing possible adverse effects on species and communities. Preliminary studies of radioecological parameters including distribution coefficients and concentration ratios, for 90Sr and 137Cs were not in full agreement with the default values used in the ERICA Tool and the RESRAD BIOTA codes. The unique radiation situation in the Techa River, which was contaminated by long-lived radionuclides (90Sr and 137Cs) in the middle of the last century allows improved knowledge about these parameters for river systems. Therefore, the study was focused on the evaluation of radioecological parameters (distribution coefficients and concentration ratios for 90Sr and 137Cs) for the Techa River and the Miass River, which is assumed as a comparison waterbody. To achieve the aim the current contamination of biotic and abiotic components of the river ecosystems was studied; distribution coefficients for 90Sr and 137Cs were calculated; concentration ratios of 90Sr and 137Cs for three fish species (roach, perch and pike), gastropods and filamentous algae were evaluated. Study results were then compared with default values available for use in the well-known computer codes ERICA Tool and RESRAD BIOTA (when site-specific data are not available). We show that the concentration ratios of 137Cs in whole fish bodies depend on the predominant type of nutrition (carnivores and phytophagous). The results presented here are useful in the context of improving of tools for assessing concentrations of radionuclides in biota, which could rely on a wider range of ecosystem information compared with the process limited the current versions of ERICA and RESRAD codes. Further, the concentration ratios of 90Sr are species-specific and strongly dependent on Ca2+ concentration in water. The universal characteristic allows us to combine the data of fish caught in the water with different mineralization by multiplying the concentration of Ca2+. The concentration ratios for fishes were well-fitted by Generalized Logistic Distribution function (GLD). In conclusion, the GLD can be used for probabilistic modeling of the concentration ratios in freshwater fishes to improve the confidence in the modeling results. This is important in the context of risk assessment and regulatory.



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Editorial board

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Publication date: June 2016
Source:Journal of Environmental Radioactivity, Volume 157





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