EFFECT OF PECTIN-TAGGED SILVER NANOCOMPOSITE ON A-72 CANCER CELL LINE

2016-08-09T05-51-53Z
Source: Indo American Journal of Pharmaceutical Research
Emmanuel U. Nwakwasi, Baisakhi Moharana, M. Parthiban, S. P. Preetha.
The pectin-tagged silver nanocomposite was explored for its antitumor properties against A-72 (Canine fibroblast tumor) cancer cell line. Pectin is a natural polysaccharide found in high amounts in most cell wall of plants. It is non-toxic in nature and functions as both intercellular and intracellular cementing material in addition to its chemo-preventive and anti-tumor activities against some aggressive, recurrent cancers. Thus, to improve the efficacy of pectin and to reduce the dose, it was conjugated into a silver nanocomposite. The synthesized silver nanocomposite, pectin powder and pectin-tagged silver nanocomposite were analyzed by high resolution scanning electron microscopy (HR-SEM), Fourier Transform Infra-Red Spectroscopy (FTIR), X-Ray Diffraction (XRD), UV-Vis Spectroscopy, zeta potential and entrapment efficiency so as to determine the size, morphology, stability and various functional groups of the composite. Their possible anticancer activity against A-72 cancer cells were further evaluated by MTT cytotoxicity Assay. The Zeta Potential analysis of the synthesized AgNPs and pectin-tagged silver nanocomposite were found to be +59.2 mV and 25.9 mV respectively which indicate a good and fair stability. Conclusively, with an average diameter of 42nm revealed by HR-SEM, the pectin-tagged silver nanocomposite exhibited a very significant cytotoxic effect on A-72 cancer cell line when compared with the standard anticancer drug (Fluorouracil/5-FU) by attaining a maximum inhibition percentage of 85.88% which is comparable to 88.88% of fluorouracil/5-FU. This clearly indicates the antitumor efficacy of the synthesized nanocomposite when conjugated with pectin and thus, proved to be a potential anticancer drug.


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