EVALUATE THE ANTIOXIDANT ACTIVITY AND FT-IR SPECTROSCOPIC ANALYSIS OF PURIFIED β-GALACTOSIDASE FROM ASPERGILLUS TERREUS

2016-08-12T04-14-36Z
Source: Indo American Journal of Pharmaceutical Research
B. Vidya, M. Palaniswamy, S. Sowmya, S. Priyanga, P. Chella Perumal, K. Devaki, V. K. Gopalakrishnan*.
Aim: To evaluate the antioxidant activity and FT-IR spectroscopic analysis of β-galactosidase from newly isolated fungal strain, Aspergillus terreus. Methods: In vitro antioxidant activity was evaluated by different assays, including reducing power assay, 2,2-diphenylpicryl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2'azinobis 3-ethylbenzothiozoline-6-sulfonic acid (ABTS+) assay, hydroxyl radical activity (OH.), hydrogen peroxide (H2O2), nitric oxide radical (NO), superoxide radical scavenging assays using ascorbic acid as a standard. The functional groups were analyzed by using FTIR spectroscopy. The FT-IR spectrum in the mid-infrared region 4000400 cm1 was used for discrimination and to categorize various functional groups present in β-galactosidase. Results: The findings indicated that the β-galactosidase posses antioxidant activities were significantly increased, comparable with that of the standard ascorbic acid, in a dose-dependent manner. Conclusion: The results revealed that β-galactosidase from newly isolated fungal strain Aspergillus terreus have promising antioxidant activity and it further exploration for their potential effective use.


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