Cryopreservation of Black Bengal buck semen by Tris-based extenders containing different levels of egg-yolk

2016-08-16T19-20-02Z
Source: Annals of Veterinary and Animal Science
Ray K, Singh LG, Sarkar B.
The study was designed to assess the cryopreservation of extended Black Bengal buck semen in Tris-egg yolkcitric acid-glucose-glycerol (TEYCGG) extender containing 4 different levels (2.5, 5, 7.5, 10%) of egg-yolk and to compare the fresh, pre-freeze and post-thaw quality of semen. Artificial Vagina (AV) method was used to collect semen from six reproductively mature, 2 1/2 to 4 years old, healthy breeding bucks. A total of forty eight ejaculates were collected once a week for eight weeks. Semen was diluted in TEYCGG extender equilibrated at 5°C for 3 h and then the French mini (0.25 ml) straws were filled with semen and frozen in liquid nitrogen (LN2). Frozen semen straws were thawed in a water bath at 37˚C for 30 seconds after 24 h of freezing for postthaw sperm evaluation. The results revealed that only progressive motility (%) of the pre-freeze (81.58±0.54 to 67.08±0.5) and frozen semen (51.46±0.75 to 39.75±0.62), which was differed significantly (P


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