Membrane Lipid Peroxidation and Redox Status in Erythrocytes of Sickle Cell Anaemia Patients Incubated in Leaf Extract of Gongronema latifolium

2016-07-15T18-11-11Z
Source: Journal of Experimental and Integrative Medicine
Paul Chidoka Chikezie.
Aim/Background: The present study sought to ascertain the capacity of ethanolic leaf extract of Gongronema latifolium to deter membrane lipid peroxidation and exert appropriate redox status in sickle erythrocytes using in vitro models. Methods: Erythrocyte malondialdehyde (MDA) concentration, membrane thiobarbituric acid reactive substances (TBARS) concentration, ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) (GSH/GSSG ratio), methaemoglobin (MetHb) concentration and NADH-methaemoglobin reductase (NADH-MR) activity were monitored in the presence of ethanolic leaf extract of G. latifolium for 15 h using spectrophotometric methods. Results: Erythrocyte MDA concentration of the control sample incubated for 15 h was within the range of 2.43 ± 0.19 3.54 ± 0.31 mmol/mL representing 45.67% increase in MDA concentration. The capacity of the extract to lower erythrocyte MDA concentration was in a concentration dependent manner. Erythrocytes incubated for 5 h in 100 mg/dL extract caused 8.59% reduction in erythrocyte membrane TBARS, whereas erythrocytes incubated for 5 h in 400 mg/dL extract gave 22.44%; p 0.05. Conclusion: The present study showed that ethanolic leaf extract of G. latifolium deterred lipid peroxidation but did not completely obliterate the propensity of the sickle erythrocytes to undergo oxidative deterioration in vitro.


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