Purpose: The aim of this study was to determine if exercise time trials (TT) of different durations would cause different levels of peripheral and central fatigue during exercise. Methods: Twelve trained subjects (11 men, one woman) performed TT lasting 3, 10, and 40 min with repetitive self-paced concentric right knee extension at 60°·s−1 on an isokinetic dynamometer. Neuromuscular function was assessed before, during, and immediately after the TT using voluntary and electrically evoked forces. Results: Maximal voluntary contraction force, evoked peak force for single stimulus, and rating of perceived exertion reached similar levels at termination of all TT. Evoked peak force for paired stimuli of 100 Hz decreased more for the 40-min TT compared with the 3-min TT (−42% ± 15% vs −37% ± 13%, P
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