Τρίτη 21 Νοεμβρίου 2017

Key amino acid residues conferring enhanced enzyme activity at cold temperatures in an Antarctic polyextremophilic {beta}-galactosidase [Genetics]

The Antarctic microorganism Halorubrum lacusprofundi harbors a model polyextremophilic β-galactosidase that functions in cold, hypersaline conditions. Six amino acid residues potentially important for cold activity were identified by comparative genomics and substituted with evolutionarily conserved residues (N251D, A263S, I299L, F387L, I476V, and V482L) in closely related homologs from mesophilic haloarchaea....

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