Antioxidant properties of ethanol and fractions of ocimum gratissimum leave in paracetamol-induced oxidative stress in Wistar albino rats.

2016-09-06T15-12-49Z
Source: Journal of Experimental and Integrative Medicine
ukpabi-ugo, Jacinta Chigozie, Patrick -Iwuanyanwu, kingsley Chukwuemeka,Monanu Michael Okechukwu.
Objective:Thepresent study is aimed at evaluating the antioxidant activity of different solvent fractions (ethylacetate, ethanol, hexane, aqueous)of Ocimumgratissimum (OG)onoxidative stress indices in Paracetamol-induced oxidative stress in Wistar albino rats. Methods: Thirty five (35) Wistar albinorats of both sexes were divided into seven groups (n=5). Paracetamol(PCM) (500mg/kg BW) dissolved in olive oil was administered daily for 28 days to induce hepatic damage in experimental rats.Control group received 1ml olive oil only. 400 mg/kg BW doses of ethyl acetate, ethanol, hexane, aqueous fractions of OG and silymarin(SIL) (100mg/kg) in addition to 500mg/kg body weight of PCM dissolved in 1ml of olive oil respectivelywere used as treatment groups daily for 28 days. Blood samples were collected by cardiac puncture and activities of Superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) using standard biochemical methods. Lipid peroxidation was estimated by measuring Malondialdehyde (MDA) concentration. Results:Superoxide dismutase (SOD) activity increased significantly (p≤ 0.05) in groups administered different fractions of OG and SIL when compared with control. Catalase (CAT) activity showed significant (p≤ 0.05) increase in ethanol and ethyl acetate fractions of OG while group treated with SIL and groups administered hexane and aqueous fractions showed a marked increase when compared with rats treated with PCM only. Reduced glutathione (GSH) level increased significantly (p≤ 0.05) in aqueous fraction while SIL-treated group and groups administered fractions of OG (ethyl acetate, ethanol and hexane) showed a marked increase when compared with PCM only treated group. Malondialdehyde concentration decreased significantly (p≤ 0.05) in groups administered different fractions of OG when compared with PCM only treated group. Conclusion:These findings are suggestive of the antioxidant properties of different solvent fractions of Ocimum gratissimum extract in paracetamol-induced oxidative stress with ethyl acetate fraction showing the highest activity. Reduced glutathione level increased significantly (p≤ 0.05) in aqueous fraction while silymarin and other fractions of OG (ethyl acetate, ethanol and hexane) showed a marked increase when compared with negative control. Malondialdehyde level decreased significantly (p≤ 0.05) in groups treated with different fractions of OG when compared with negative control.Histopathological examination of the liver sections corroborated the data from biochemical studies. Conclusion: These findings indicate that different solvent fractions of Ocimum gratissimum extract may possess antioxidant properties and thus reduced the oxidative stress that was caused by paracetamol overdose with ethyl acetate fraction being the more effective fraction.


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