Parallel processing of afferent olfactory sensory information

Abstract

Primary olfactory receptor neurons terminate in anatomically and functionally discrete cortical modules known as olfactory bulb glomeruli. The synaptic connectivity and postsynaptic responses of mitral and external tufted cells within the glomerulus may involve both direct and indirect components. For example, it has been suggested that sensory input to mitral cells is indirect through feedforward excitation from external tufted cells. We also observed feedforward excitation of mitral cells with weak stimulation of the olfactory nerve layer, however, focal stimulation of an axon bundle entering an individual glomeruli, revealed that mitral cells receive monosynaptic afferent inputs. Although external tufted cells had a 4.1 fold larger peak EPSC amplitude, integration of the evoked currents showed that the synaptic charge was 5 fold larger in mitral cells, reflecting the prolonged response in mitral cells. Presynaptic afferents onto mitral and external tufted cells had similar quantal amplitude and release probability, suggesting that the larger peak EPSC in external tufted cells resulted from more synaptic contacts. Our results indicate that the monosynaptic afferent input to mitral cells depends on the strength of odorant stimulation. The enhanced spiking we observed in response to brief afferent input provides a mechanism to amplify sensory information and contrasts with the transient response in external tufted cells. These parallel input paths may have discrete functions in processing olfactory sensory input.

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