Assessment of anti-inflammatory and anti-arthritic properties of Acmella uliginosa (Sw.) Cass. based on experiments in arthritic rat models and qualitative GC/MS analyses.

2016-05-30T06-00-19Z
Source: Journal of Intercultural Ethnopharmacology
Subhashis Paul, Sudeb Sarkar, Tanmoy Dutta, Soumen Bhattacharjee.
AIM: The principle objective of the study was to explore the anti-arthritic properties of Acmella uliginosa (Sw.) Cass. flower in a rat model and to identify potential anti-inflammatory compounds derived from flower extracts. The synergistic role played by a combination of Acmella uliginosa flower and Aloe vera gel crude extracts was also investigated. METHOD: Male Wister rats induced with Freunds Complete Adjuvant (FCA) were used as a disease model of arthritic paw swelling. There were three experimental and two control groups each consisting of 5 rats. Paw circumference and serum biochemical parameters were evaluated to investigate the role of the flower extracts in disease amelioration through a feeding schedule spanning 21 days. GC/MS analyses were performed to search for the presence of anti-inflammatory compounds in the ethanolic and n-hexane solvent extracts of the flower. RESULTS: As a visual cue to the experimental outcomes, FCA-induced paw swelling decreased to the normal level; and haemoglobin, serum protein, and albumin levels were significantly increased in the treated animals. The creatinine level was estimated to be normal in the experimental rats after the treatment. The combination of Acmella uliginosa and Aloe vera showed the best recovery potential in all the studied parameters, confirming the synergistic efficacy of the herbal formulation. GC/MS analyses revealed the presence of at least 5 anti-inflammatory compounds including 9-Octadecenoic acid (Z)-,phenylmethyl ester, astaxanthin, à-N-Normethadol, fenretinide, that have reported anti-inflammatory/anti-arthritic properties. CONCLUSION: Our findings indicated that the crude flower homogenate of Acmella uliginosa contains potential anti-inflammatory compounds which could be used as an anti-inflammatory/anti-arthritic medication.


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