Coagulation Gene Expression Profiling in Infants with Necrotizing Enterocolitis.

Objectives: Coagulopathy and mesenteric thrombosis are common in premature neonates with necrotizing enterocolitis (NEC). This pilot study aimed to investigate the hypothesis that there are changes in the gene expression related to the coagulation and anticoagulation systems in NEC. Methods: Consecutive neonates (n = 11) with NEC (Bell's stages 2-3) were recruited. Two comparison groups, matched for birth weight and corrected gestational age, were selected based on: the absence of inflammation and coagulopathy (healthy control, n = 10); or the presence of a confirmed blood infection (sepsis control, n = 12). A pathway specific quantitative PCR array was used to determine the expression of 94 genes involved in human blood coagulation and anticoagulation cascade. Results: Twelve genes of the coagulation and anticoagulation systems were significantly altered in the NEC patients compared to healthy controls. In particular, ELANE, CD63, PROS1, HGF, F12 were significantly up-regulated (mean fold changes +2.74, p

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