Role of recombinant SXP/RAL-2 family protein Wuchereria bancrofti L2 (rWbL2) as vaccine candidate in lymphatic filariasis in mastomys

2016-03-28T07-32-44Z
Source: International Journal of Research in Medical Sciences
Dhananjay Andure, M. V. R. Reddy, Kiran Pote, Ramchandra Padalkar, Vishal Khatri, Nitin Amdare.
Background: Lymphatic filariasis is caused mainly by three lymph dwelling parasites; Wuchereria bancrofti, Brugia malayi and Brugia timori. It remains main cause of clinical morbidity in tropical and sub-tropical countries. Methods: In the present study, we evaluated the immunogenicity and efficacy of recombinant SXP/RAL-2 family protein, rWbL2 to induce protective immune response against B. malayi infection. Mastomys were immunized intraperitoneally with rWbL2 antigen and their ability to induce humoral response, cell mediated immune response and protective efficacy was evaluated. Results: The mastomys, which are fully permissible rodents for Brugia malayi when immunized with rWbL2 could induce 51.25% in situ cytotoxicity against Brugia malayi infective (L3) larvae. We also seen that, it is characterized by higher levels of WbL2- specific IgG1 and IgG2a antibodies and increased levels of IFN-γ, IL-4 and IL-10 cytokines production by the spleen cells. Conclusions: The findings suggest that cellular as well as humoral immune response in the immunized mastomys and may represent effective vaccine candidate for filariasis.


from Scope via xlomafota13 on Inoreader http://ift.tt/1Ux0pbw
via IFTTT