Experiments were performed on three groups of male Sprague–Dawley rats, each of six animals. Group 1 consisted of intact animals; group 2 of animals given zinc paste, and group 3 of animals given fraction 3 of antiseptic Dorogov's stimulator (ASD-3) by application to the skin on the back. Detection of c-Fos-positive neurons in different parts of the brain and studies of behavioral responses demonstrated activation of neurons in the cingulate, motor, and piriform areas of the cerebral cortex and the striatum, with increases in motor and ultrasound activity; there was a correlation between behavioral reactions and activation of neurons in these brain areas. These results provide evidence of the concomitant regulation of behavior by multiple brain structures and that ASD-3 has neurotropic properties.
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