The morphometric characteristics and distribution of Clarke's nuclei in the gray matter of the spinal cord were studied in cats by histochemical detection of acetylcholinesterase, an immunohistochemical marker for non-phosphorylated domains of neurofilament heavy chains (SMI-32) and the Klüver–Barrera method for detection of myelinated fibers. Results averaged for five cats yielded metric maps of segments LI–IV of the spinal cord with superimposed spatial coordinates of the boundaries of Clarke's nuclei. These studies provide the information required for more precise stereotaxic access to Clarke's nuclei for investigations in future research and applied studies.
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