ISOLATION AND PHARMACOLOGICAL STUDIES OF KARANJACHROMENE FROM THE SEEDS OF PONGAMIA PINNATA (L. PIERRE)

2016-09-11T23-14-43Z
Source: International Journal of Current Research and Review
Devendra N. Kage, Nuzhahat Tabassum, Vijaykumar B. Malashetty, Raghunandan Deshpande, Y. N. Seetharam.
Context: Isolation of chemical compound karanjachromene from the Seeds of Pongamia Pinnata and evaluation of its anti-inflammatory and analgesic activities. Materials and methods: Karanjachromene has been successfully extracted from the seeds of Pongamia Pinnata using n-hexane, petroleum ether and alcohol with Soxhlet extraction. Anti-inflammatory and analgesic activities of the some were assessed administering in Swiss albino mice. The anti-inflammatory activity of the test compound was determined by mice paw edema inhibition method. The analgesic activity was determined by both acetic acid induced writhing and tail immersion methods. Results: Karanjachromene at doses 25 mg/kg and 50 mg/kg shown 40.48% and 59.6% inhibition of paw edema respectively, at the end of 3 h standard drug diclofenac sodium produced 63.01% inhibition in paw volume at 10 mg/kg. The oral administration of test compound karanjachromene significantly inhibited writhing response induced by acetic acid in a dose dependent manner. Karanjachromene produced 29.64% and 42.14% inhibition of writhing at doses 25 mg/kg and 50 mg/kg respectively. Standard drug diclofenac sodium produced 56.47 % inhibition of writhing at 10 mg/kg. Discussion and Conclusion: The administration of karanjachrome is potent to inhibit the paw edema starting from the 1st hour and during all phases of inflammation, which may be due to inhibition of different inflammatory mediators. The acetic acid induced writhing response could be mediated by peritoneal mast cells, acid sensing ion channels and the prostaglandin pathways. The test compound inhibited both mechanisms of pain and inflammation and are more found active peripherally than centrally. Karanjachromene exhibited significant anti-inflammatory and analgesic agents


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