Study of analgesic effect of amiloride compared to aspirin in chemically induced pain models in rats and mice

2016-06-26T00-48-19Z
Source: National Journal of Physiology, Pharmacy and Pharmacology
Bhanu Prakash Kolasani, Raghunatha Rao Ponnaluri, Raghunandan Mudium.
Background: Acid-sensing ion channels (ASIC) contribute to acid-evoked pain in many painful inflammatory and ischemic conditions such as rheumatoid arthritis, cardiac ischemia, and exhausted skeletal muscles, which are accompanied by local tissue acidosis. Amiloride, a potassium-sparing diuretic was recently discovered to be a blocker of these ASIC. Aims and Objectives: The objective of present study is to evaluate the analgesic activity of amiloride in chemically induced pain models of rats and mice and compare it against the standard analgesic aspirin. Materials and Methods: A total of 24 mice and 24 rats were distributed into four groups of 6 each: Group A received distilled water as control, Group B received the test drug amiloride - low dose (20 mg/kg), Group C received amiloride-high dose (40 mg/kg), and Group D received standard drug aspirin (25 mg/kg); all drugs were given intraperitoneally. In formalin test, 0.025 ml of 1% formalin was injected under the plantar surface of hind paw and the mean time of paw licking (MTPL) was measured in both early (0-5 min) as well as late (20-40 min) phase. In acetic acidinduced writhing test, the animals were injected with 1 ml/100 g body weight of 0.6% acetic acid i.p. The number of writhings produced was recorded over a period of 20 min. Results: In the early phase of formalin test, the MTPL was significantly reduced (P

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