Investigation of the In Vitro Antioxidant and Cytotoxic Activities of Xanthosoma sagittifolium Leaf

2016-06-19T02-57-36Z
Source: Indo American Journal of Pharmaceutical Research
Md. Sarwar Hossain*, Muhammad Asaduzzaman, Md. Sahab Uddin, Md. Ali Asif Noor, Md. Ashikur Rahman, Mst. Shirajum Munira, Md. Asaduzzaman..
Plants are store house of phytochemical constituents used to diseases from the ancient era. The hunt for new remedies from natural origin is still going on to discover noble therapy against new and existence diseases. In this study ethyl acetate fraction (EAF), n-Hexane fraction (NHF), chloroform fraction (CLF) and aqueous fraction (AQF) of Xanthosoma sagittifolium (XS) leaf have been investigated for the evaluation of antioxidant and cytotoxic activities. For determination of antioxidant and cytotoxic activities total antioxidant capacity (TAC), total phenolic content (TPC), reducing power activity (RPA), DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging activity and brine shrimp lethality (BSL) assay were performed respectively. TFC of EAF (54.44 mg/g GAE) was found significantly higher as compared to other solvent fractions. Among four different fractions CLF showed the highest total antioxidant activity at 600 μg/ ml concentration followed by EAF, NHF and AQF. The ranking order for RPA was EAF > AQF > CLF > NHF. The IC50 values for the DPPH radical scavenging test were in the order of EAF (52.16 μg/ml) > CLF (99.78  0.24) > AQF (190.73  1.11) > NHF (394.01  10.49). Among all extracts of XS, NHF showed the highest cytotoxicity activity with LD50 value of 24.00 g/ml and the AQF showed the lowest cytotoxicity activity with LD50 value of 39.48 g/ml respectively. Our study suggests that XS leaf have strong antioxidant and cytotoxic effect confirming the traditional uses of this plant and different opportunities have been exposed that will lead further research.


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