In vitro effects of nicotine on the non-small-cell lung cancer line A549

2016-04-05T17-31-03Z
Source: Journal of the Pakistan Medical Association
Tao Gao, Xue-Liang Zhou, Sheng Liu, Chang-Xiu Rao, Wen Shi, Ji-Chun Liu.
Objective: To investigate in vitro effects of nicotine on the non-small-cell lung cancer line A549. Methods: The case-control study was conducted at the First Affiliated Hospital of Nanchang University from 1st January to 30th June, 2014 and comprised A549 cells which were treated with a series of concentrations of nicotine (0.01 µM, 0.1 µM, 1 µM and 10 µM) for 24 hours. Control cells were incubated under the same conditions without the addition of nicotine. Cell growth was detected by monotetrazolium salt [3-(4, 5-dimethyl-2-thiazolyl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay. Cell apoptosis was detected by Haematoxylin and Eosin staining, immunofluorescence analysis of Filamentous actin and electron microscope observation. Results: Nicotine had no significant effect on A549 cell growth at the dose of 0.01µM (p>0.05), but had significant growth inhibitory effects at the doses of 0.1µM, 1µM and 10µM (p


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