Construction and Validation of a Dual-Transgene Vector System for Stable Transformation in Plants

Publication date: Available online 10 March 2016
Source:Journal of Genetics and Genomics
Author(s): Zhimin He, Bin Liu, Xu Wang, Mingdi Bian, Reqing He, Jindong Yan, Ming Zhong, Xiaoying Zhao, Xuanming Liu
In this study, we constructed dual-transgene vectors (pDT1, pDT7, and pDT7G) that simultaneously co-expressed two genes for use in plants, and ACTIN2 and UBQ10 promoters were used to control the expression of these two genes. The use of 4×Myc, 3×HA, and 3×Flag reporter genes allowed for the convenient identification of a tunable co-expression system in plants, whereas the dexamethasone (Dex) inducible reporter gene c-terminus of the glucocorticoid receptor (cGR) provided Dex-dependent translocation of the fusion gene between the nucleus and cytoplasm. The function of pDT vectors was validated using four pairwise genes in Nicotiana benthamiana or Arabidopsis thaliana. The co-expression efficiency of two genes from the pDT1 and pDT7G vectors was 35% and 42%, respectively, which ensured the generation of sufficient transgenic materials. These pDT vectors are simple, reliable, efficient, and time-saving tools for the co-expression of two genes through a single transformation event and can be used in the study of protein-protein interactions or multi-component complexes.



from Genetics via xlomafota13 on Inoreader http://ift.tt/1phJnRE
via IFTTT